Strukturierung von artikulärem Knorpel

Abstract

Background: Cartilage defects caused by injuries or osteoarthritis are a common problem. As cartilage tissue hardly regenerates itself, damage can lead to joint destruction in the long term. In view of increasing life expectancy, innovative treatment approaches are essential, as current therapies such as physiotherapy or surgical interventions often only have limited success. Research into new methods in tissue engineering is therefore of great importance. Methods: This work systematically investigates the interaction between hyaline cartilage tissue and a CO2 laser. The aim is to demonstrate and predict its potential for cartilage structuring. Standardized cartilage samples were structured with a CO₂ laser using holes and lines as irradiation shapes. Laser parameters such as power, speed and number of repetitions were systematically varied. The effects on the cartilage matrix and possible cell damage were then analyzed microscopically. Results: Laser settings were determined that showed precise and fine control of the patterning depth. Selective structuring without penetrating the cartilage was achieved with 30 watt laser power, 21.3 cm/s speed and increasing repetition rates (D1 - 5). These settings also led to an increase in surface area and optimal device protection. Conclusion and Discussion: The work concludes that targeted laser settings lead to predictable, systemic effects. Precise and controlled structuring plays a crucial role in the processing of hyaline cartilage. Further research is needed to establish a standardized approach and to understand longterm cellular responses and biocompatibility, which will provide a basis for clinical application.

Date of Award	2025
Original language	German (Austria)
Supervisor	Jaroslaw Jacak (Supervisor)