Optimization of Functional Control for a Multiplex Micro ELISA Point-of-Care Test System

  • Maximilian Ratzberger

Student thesis: Master's Thesis

Abstract

This master's thesis investigates various methods for improving the functional control of the Genspeed R2 analysis system, a microfluidic ELISA system specifically designed for Point-of-Care use. The focus is on verifying the fluidic paths and accurately determining the dispensed volume of reagents. The goal is to develop a systematic process that allows the system to reliably detect unforeseen volume reductions or blockages in the fluidic paths. To assess the functional control of the Genspeed R2 analysis system, two methods were employed: First, the optical behaviour of a liquid droplet on a solid surface was examined. The droplet acts like a lens, concentrating the light, which can then be detected via absorption measurement. Additionally, this method was modified to calculate the dispensed volume of the droplet. Second, the chemiluminescence signal was analysed, focusing on the reduction of the signal due to the dilution of the reagent concentration. This dilution occurs through the addition of the washing solution, which dilutes the reagent responsible for the chemiluminescence, allowing for the measurement of signal changes. For both methods, a repeatable process was developed and experimentally tested. The results show that it is possible to detect a blocked path with both methods. Additionally, Method 1 demonstrated a correlation between the detected signal and the dispensed droplet volume. However, Method 1 proves to be more error-prone, as even small contaminations on the rinsing chip can lead to measurement inaccuracies. In contrast, Method 2 exhibits greater stability and reliability, as the chemiluminescence signal is less susceptible to such disturbances and delivers more consistent results. Therefore, Method 2 should be further developed as the standard method to ensure reliable functional control. Nevertheless, it would be beneficial to improve the reliability of Method 1, as its ability to provide precise volume determination could be advantageous in certain applications.
Date of Award2024
Original languageEnglish
SupervisorBirgit Plochberger (Supervisor)

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