Charakterisierung der Komplementbindung modifizierter Antikörper mittels Quarzkristallmikrowaage

Abstract

Antibody-based therapies have established themselves as a promising method for treating a wide variety of conditions, such as autoimmune diseases, chronic inflammatory diseases and tumours. The knowledge of humoral immunology and findings in the field of antibody engineering have contributed to the development of new therapeutic antibodies. The activation of the complement system by antibody-antigen complexes plays a decisive role in this process. In this study, Fc-modified IgG antibodies (IgG1 – IgG4) are characterised regarding their complement binding ability, using a quartz crystal microbalance. A comparison with the wild-type variants shows that these modified antibodies exhibit different complement binding and oligomerisation behaviour, which may be helpful in the development of therapeutic antibody formats.

Date of Award	2025
Original language	German (Austria)
Awarding Institution	FH Gesundheitsberufe OÖ GmbH
Supervisor	Andreas Karner (Supervisor)