Biocompatibility testing of 2D structured polymer surfaces

Abstract

The aim of this project was to investigate cell morphology, viability, and proliferation on silanized glass substrates coated with conductive chemical polymers. While silanized surfaces offer improved stability and adhesion compared to bare cover glass, their chemical components are often not inherently biocompatible. Therefore, surface modification was essential to reduce cytotoxicity while preserving conductivity and ensuring the structural integrity of the scaffolds under biological conditions. Achieving a balance between biocompatibility and conductivity is particularly important for applications such as two-photon lithography, where nanostructures can be directly fabricated on biocompatible surfaces. These conductive scaffolds have the potential to support and guide the growth of specific cell types. In this study, HeLa cells, chosen for their relative robustness, were used to assess the initial biocompatibility of the modified surfaces. The long-term goal is to engineer scaffolds suitable for more sensitive cell types, particularly neurons and muscle cells, which respond to electrical stimuli. By enabling precise patterning and electrical stimulation, these materials may one day facilitate the formation of functional neural networks, advancing the fields of tissue engineering and regenerative medicine. Although this study is preliminary, it offers valuable insights into conductive polymers and silanized substrates to enhance cellular compatibility, thereby laying the groundwork for future biomedical applications.

Date of Award	2025
Original language	English
Supervisor	Jaroslaw Jacak (Supervisor)