Wood-based lignans and polyphenols improve intestinal health by strengthening barrier integrity and reducing inflammation

Mara Heckmann; Nadiia Sadova; Ivana Drotárová; Stefanie Atzmüller; Bettina Schwarzinger; Roberto Mauricio Carvalho Guedes; Paula Angelica Correia; Stefan Hirtenlehner; Christine Potthast; Gerald Klanert; Julian Weghuber

Wood-based lignans and polyphenols improve intestinal health by strengthening barrier integrity and reducing inflammation

Mara Heckmann, Nadiia Sadova, Ivana Drotárová, Stefanie Atzmüller, Bettina Schwarzinger, Roberto Mauricio Carvalho Guedes, Paula Angelica Correia, Stefan Hirtenlehner, Christine Potthast, Gerald Klanert, Julian Weghuber

Research output: Contribution to conference › Abstract › peer-review

Abstract

Lignans and polyphenols derived from wood are known to exhibit a broad spectrum of biological activities, indicating promising potential as relevant constituents of feed supplements. We aimed to investigate two extracts derived from wood lignan-based feed additives (‘ROI’ and ‘Protect’) regarding their impact on selected parameters of intestinal functionality. The antioxidant and anti-inflammatory properties of the extracts were determined by measuring the effects on reactive oxygen species (ROS) and pro-inflammatory cytokine production in-vitro. The impact on intestinal barrier integrity was evaluated in Caco-2 cells and in Drosophila melanogaster by documenting leaky gut formations. Furthermore, feeding trials with ROI and Protect using chickens and infected piglets, respectively, were conducted. Intestinal samples of the chickens were taken to study gene expression of pro-inflammatory target genes by qPCR. Oxidative lesions recovered from the intestine of infected piglets were analyzed for the levels of superoxide dismutase, glutathione and lipid peroxidation. Protect lowered ROS production in Caco-2 cells and reversed the stress-induced weakening of barrier integrity. ROI inhibited the expression or secretion of interleukin-8 (IL-8), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNFα) in THP-1 macrophages. Moreover, ROI decreased leaky gut formation and mortality rates in Drosophila melanogaster. ROI-fed chickens showed decreased gene expression levels for IL-1β and interleukin-8-like 2 (IL-8L2). Dietary supplementation with Protect improved the antioxidant status and barrier integrity of the intestines of infected piglets. In conclusion, wood lignan-enriched feed supplements are valuable tools that support intestinal health by exerting antioxidant, anti-inflammatory and barrier-strengthening effects. Previously published in: Heckmann et al. (2022) Molecules 27, 6327.

Original language	English (American)
Publication status	Published - 11 Jul 2023
Event	The 47th FEBS Congress - The Palais des Congrès de Tours , Tours, France Duration: 8 Jul 2023 → 12 Jul 2023 https://2023.febscongress.org

Conference

Conference	The 47th FEBS Congress
Abbreviated title	FEBS 2023
Country/Territory	France
City	Tours
Period	08.07.2023 → 12.07.2023
Internet address	https://2023.febscongress.org

Cite this

@conference{76b44e01404e4ead8c604293379eb676,

title = "Wood-based lignans and polyphenols improve intestinal health by strengthening barrier integrity and reducing inflammation",

abstract = "Lignans and polyphenols derived from wood are known to exhibit a broad spectrum of biological activities, indicating promising potential as relevant constituents of feed supplements. We aimed to investigate two extracts derived from wood lignan-based feed additives ({\textquoteleft}ROI{\textquoteright} and {\textquoteleft}Protect{\textquoteright}) regarding their impact on selected parameters of intestinal functionality. The antioxidant and anti-inflammatory properties of the extracts were determined by measuring the effects on reactive oxygen species (ROS) and pro-inflammatory cytokine production in-vitro. The impact on intestinal barrier integrity was evaluated in Caco-2 cells and in Drosophila melanogaster by documenting leaky gut formations. Furthermore, feeding trials with ROI and Protect using chickens and infected piglets, respectively, were conducted. Intestinal samples of the chickens were taken to study gene expression of pro-inflammatory target genes by qPCR. Oxidative lesions recovered from the intestine of infected piglets were analyzed for the levels of superoxide dismutase, glutathione and lipid peroxidation. Protect lowered ROS production in Caco-2 cells and reversed the stress-induced weakening of barrier integrity. ROI inhibited the expression or secretion of interleukin-8 (IL-8), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNFα) in THP-1 macrophages. Moreover, ROI decreased leaky gut formation and mortality rates in Drosophila melanogaster. ROI-fed chickens showed decreased gene expression levels for IL-1β and interleukin-8-like 2 (IL-8L2). Dietary supplementation with Protect improved the antioxidant status and barrier integrity of the intestines of infected piglets. In conclusion, wood lignan-enriched feed supplements are valuable tools that support intestinal health by exerting antioxidant, anti-inflammatory and barrier-strengthening effects. Previously published in: Heckmann et al. (2022) Molecules 27, 6327.",

author = "Mara Heckmann and Nadiia Sadova and Ivana Drot{\'a}rov{\'a} and Stefanie Atzm{\"u}ller and Bettina Schwarzinger and Guedes, {Roberto Mauricio Carvalho} and Correia, {Paula Angelica} and Stefan Hirtenlehner and Christine Potthast and Gerald Klanert and Julian Weghuber",

year = "2023",

month = jul,

day = "11",

language = "Englisch (Amerika)",

note = "The 47th FEBS Congress ; Conference date: 08-07-2023 Through 12-07-2023",

url = "https://2023.febscongress.org",

}

TY - CONF

T1 - Wood-based lignans and polyphenols improve intestinal health by strengthening barrier integrity and reducing inflammation

AU - Heckmann, Mara

AU - Sadova, Nadiia

AU - Drotárová, Ivana

AU - Atzmüller, Stefanie

AU - Schwarzinger, Bettina

AU - Guedes, Roberto Mauricio Carvalho

AU - Correia, Paula Angelica

AU - Hirtenlehner, Stefan

AU - Potthast, Christine

AU - Klanert, Gerald

AU - Weghuber, Julian

PY - 2023/7/11

Y1 - 2023/7/11

N2 - Lignans and polyphenols derived from wood are known to exhibit a broad spectrum of biological activities, indicating promising potential as relevant constituents of feed supplements. We aimed to investigate two extracts derived from wood lignan-based feed additives (‘ROI’ and ‘Protect’) regarding their impact on selected parameters of intestinal functionality. The antioxidant and anti-inflammatory properties of the extracts were determined by measuring the effects on reactive oxygen species (ROS) and pro-inflammatory cytokine production in-vitro. The impact on intestinal barrier integrity was evaluated in Caco-2 cells and in Drosophila melanogaster by documenting leaky gut formations. Furthermore, feeding trials with ROI and Protect using chickens and infected piglets, respectively, were conducted. Intestinal samples of the chickens were taken to study gene expression of pro-inflammatory target genes by qPCR. Oxidative lesions recovered from the intestine of infected piglets were analyzed for the levels of superoxide dismutase, glutathione and lipid peroxidation. Protect lowered ROS production in Caco-2 cells and reversed the stress-induced weakening of barrier integrity. ROI inhibited the expression or secretion of interleukin-8 (IL-8), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNFα) in THP-1 macrophages. Moreover, ROI decreased leaky gut formation and mortality rates in Drosophila melanogaster. ROI-fed chickens showed decreased gene expression levels for IL-1β and interleukin-8-like 2 (IL-8L2). Dietary supplementation with Protect improved the antioxidant status and barrier integrity of the intestines of infected piglets. In conclusion, wood lignan-enriched feed supplements are valuable tools that support intestinal health by exerting antioxidant, anti-inflammatory and barrier-strengthening effects. Previously published in: Heckmann et al. (2022) Molecules 27, 6327.

AB - Lignans and polyphenols derived from wood are known to exhibit a broad spectrum of biological activities, indicating promising potential as relevant constituents of feed supplements. We aimed to investigate two extracts derived from wood lignan-based feed additives (‘ROI’ and ‘Protect’) regarding their impact on selected parameters of intestinal functionality. The antioxidant and anti-inflammatory properties of the extracts were determined by measuring the effects on reactive oxygen species (ROS) and pro-inflammatory cytokine production in-vitro. The impact on intestinal barrier integrity was evaluated in Caco-2 cells and in Drosophila melanogaster by documenting leaky gut formations. Furthermore, feeding trials with ROI and Protect using chickens and infected piglets, respectively, were conducted. Intestinal samples of the chickens were taken to study gene expression of pro-inflammatory target genes by qPCR. Oxidative lesions recovered from the intestine of infected piglets were analyzed for the levels of superoxide dismutase, glutathione and lipid peroxidation. Protect lowered ROS production in Caco-2 cells and reversed the stress-induced weakening of barrier integrity. ROI inhibited the expression or secretion of interleukin-8 (IL-8), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNFα) in THP-1 macrophages. Moreover, ROI decreased leaky gut formation and mortality rates in Drosophila melanogaster. ROI-fed chickens showed decreased gene expression levels for IL-1β and interleukin-8-like 2 (IL-8L2). Dietary supplementation with Protect improved the antioxidant status and barrier integrity of the intestines of infected piglets. In conclusion, wood lignan-enriched feed supplements are valuable tools that support intestinal health by exerting antioxidant, anti-inflammatory and barrier-strengthening effects. Previously published in: Heckmann et al. (2022) Molecules 27, 6327.

M3 - Abstract

T2 - The 47th FEBS Congress

Y2 - 8 July 2023 through 12 July 2023

ER -