Visualization of vesicle transport along and between distinct pathways in neurites of living cells.

GJ Schütz, M Axmann, S Freudenthaler, H Schindler, K Kandror, JC Roder, A Jeromin

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Trafficking of secretory vesicles along neurites of PC12 cells was visualized by 2D and 3D real-time imaging using fluorescence microscopy. Vesicle motion along distinct pathways was directly seen. From an overlay of individual pathways, the underlying cytoskeletal filament could be imaged at a subwavelength resolution. Continuous vesicle transport was interrupted by periods of diffusive motion with concomitant pathway changes. Statistical analysis shows that such interruptions were distributed stochastically along the filament, indicating a limited processivity of motor proteins also in a cellular context. Periods of diffusive motion facilitated the interaction with actively transported vesicles. Frequent associations and dissociations of vesicles have been observed consistently, pointing to a functional relevance of vesicle cotransport.

Original languageEnglish
Pages (from-to)159-167
Number of pages9
JournalMicroscopy Research and Technique
Volume63
Issue number3
DOIs
Publication statusPublished - 15 Feb 2004

Keywords

  • 3D imaging
  • Fluorescence microscopy
  • Microtubule
  • Motor molecules
  • Vesicle sorting
  • Vesicle tracking
  • Green Fluorescent Proteins
  • Rats
  • PC12 Cells
  • Protein Transport
  • Animals
  • Luminescent Proteins/genetics
  • Neurites/metabolism
  • Microscopy, Fluorescence/methods

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