The time-dependent evolution of epitope-paratope bond stability is studied by dynamic force spectroscopy. We show a significant increase in binding strength within seconds of initial association, corresponding to an early stage of bond maturation. A model linking this process to the dynamic lateral movement of antibodies on structured epitope lattices, recently studied with High-Speed AFM, is proposed. Antibodies show a bipedal walking behavior with step periods of ~40ms on such lattices, and our results indicate that these rapid cycles of association and dissociation interrupt the process of bond maturation. Consequently, epitope-paratope interactions are perpetually trapped in a weakly bound state and cannot transition into the fully docked state.
|Title of host publication||XVIII LINZ WINTER WORKSHOP 2016, Linz|
|Publisher||Johannes Kepler Universität Linz|
|Publication status||Published - 2016|
|Event||XVIII LINZ WINTER WORKSHOP 2016 - Linz, Austria|
Duration: 29 Jan 2016 → 2 Feb 2016
|Conference||XVIII LINZ WINTER WORKSHOP 2016|
|Period||29.01.2016 → 02.02.2016|