Time-evolution of epitope-paratope bonds investigated by dynamic force spectroscopy

Jürgen Strasser, Andreas Karner, Hermann Gruber, Peter Hinterdorfer, Johannes Preiner

Research output: Chapter in Book/Report/Conference proceedingsConference contribution

Abstract

The time-dependent evolution of epitope-paratope bond stability is studied by dynamic force spectroscopy. We show a significant increase in binding strength within seconds of initial association, corresponding to an early stage of bond maturation. A model linking this process to the dynamic lateral movement of antibodies on structured epitope lattices, recently studied with High-Speed AFM, is proposed. Antibodies show a bipedal walking behavior with step periods of ~40ms on such lattices, and our results indicate that these rapid cycles of association and dissociation interrupt the process of bond maturation. Consequently, epitope-paratope interactions are perpetually trapped in a weakly bound state and cannot transition into the fully docked state.
Original languageEnglish
Title of host publicationXVIII LINZ WINTER WORKSHOP 2016, Linz
PublisherJohannes Kepler Universität Linz
Publication statusPublished - 2016
EventXVIII LINZ WINTER WORKSHOP 2016 - Linz, Austria
Duration: 29 Jan 20162 Feb 2016

Conference

ConferenceXVIII LINZ WINTER WORKSHOP 2016
CountryAustria
CityLinz
Period29.01.201602.02.2016

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