The conduit system transports soluble antigens from the afferent lymph to resident dendritic cells in the T cell area of the lymph node

Michael Sixt, Nobuo Kanazawa, Manuel Selg, Thomas Samson, Gunnel Roos, Dieter P Reinhardt, Reinhard Pabst, Manfred B Lutz, Lydia Sorokin

Research output: Contribution to journalArticlepeer-review

607 Citations (Scopus)

Abstract

Resident dendritic cells (DC) within the T cell area of the lymph node take up soluble antigens that enter via the afferent lymphatics before antigen carrying DC arrive from the periphery. The reticular network within the lymph node is a conduit system forming the infrastructure for the fast delivery of soluble substances from the afferent lymph to the lumen of high endothelial venules (HEVs). Using high-resolution light microscopy and 3D reconstruction, we show here that these conduits are unique basement membrane-like structures ensheathed by fibroblastic reticular cells with occasional resident DC embedded within this cell layer. Conduit-associated DC are capable of taking up and processing soluble antigens transported within the conduits, whereas immigrated mature DC occur remote from the reticular fibers. The conduit system is, therefore, not a closed compartment that shuttles substances through the lymph node but represents the morphological equivalent to the filtering function of the lymph node.

Original languageEnglish
Pages (from-to)19-29
Number of pages11
JournalImmunity
Volume22
Issue number1
DOIs
Publication statusPublished - Jan 2005

Keywords

  • Animals
  • Antigen Presentation
  • Biological Transport
  • Biomarkers
  • CD11b Antigen/metabolism
  • CD11c Antigen/metabolism
  • Cell Adhesion
  • Dendritic Cells/metabolism
  • Fluorescent Antibody Technique
  • Injections, Subcutaneous
  • Laminin/metabolism
  • Lymph Nodes/cytology
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Video
  • Molecular Weight
  • Reticulin/metabolism
  • T-Lymphocytes/cytology

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