T cell activation is determined by the number of presented antigens.

J Deeg; M Axmann; J Matic; A Liapis; D Depoil; J Afrose; S Curado; ML Dustin; JP Spatz

doi:10.1021/nl403266t

T cell activation is determined by the number of presented antigens.

J Deeg, M Axmann, J Matic, A Liapis, D Depoil, J Afrose, S Curado, ML Dustin, JP Spatz

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113 Citations (Scopus)

Abstract

Antigen recognition is a key event during T cell activation. Here, we introduce nanopatterned antigen arrays that mimic the antigen presenting cell surface during T cell activation. The assessment of activation related events revealed the requirement of a minimal density of 90-140 stimulating major histocompatibility complex class II proteins (pMHC) molecules per μm ². We demonstrate that these substrates induce T cell responses in a pMHC dose-dependent manner and that the number of presented pMHCs dominates over local pMHC density.

Original language	English
Pages (from-to)	5619-5626
Number of pages	8
Journal	Nano Letters
Volume	13
Issue number	11
DOIs	https://doi.org/10.1021/nl403266t
Publication status	Published - 13 Nov 2013

Keywords

Nanopattern
T cell activation
TCR microclusters
artificial antigen-presenting cell
immunological synapse
nanostructures
Humans
Synapses/immunology
Major Histocompatibility Complex/immunology
Membrane Proteins/immunology
Lymphocyte Activation/immunology
Nanoparticles/chemistry
Antigen-Presenting Cells/chemistry
T-Lymphocytes/chemistry

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10.1021/nl403266t

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title = "T cell activation is determined by the number of presented antigens.",

abstract = "Antigen recognition is a key event during T cell activation. Here, we introduce nanopatterned antigen arrays that mimic the antigen presenting cell surface during T cell activation. The assessment of activation related events revealed the requirement of a minimal density of 90-140 stimulating major histocompatibility complex class II proteins (pMHC) molecules per μm 2. We demonstrate that these substrates induce T cell responses in a pMHC dose-dependent manner and that the number of presented pMHCs dominates over local pMHC density.",

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author = "J Deeg and M Axmann and J Matic and A Liapis and D Depoil and J Afrose and S Curado and ML Dustin and JP Spatz",

year = "2013",

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doi = "10.1021/nl403266t",

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journal = "Nano Letters",

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TY - JOUR

T1 - T cell activation is determined by the number of presented antigens.

AU - Deeg, J

AU - Axmann, M

AU - Matic, J

AU - Liapis, A

AU - Depoil, D

AU - Afrose, J

AU - Curado, S

AU - Dustin, ML

AU - Spatz, JP

PY - 2013/11/13

Y1 - 2013/11/13

N2 - Antigen recognition is a key event during T cell activation. Here, we introduce nanopatterned antigen arrays that mimic the antigen presenting cell surface during T cell activation. The assessment of activation related events revealed the requirement of a minimal density of 90-140 stimulating major histocompatibility complex class II proteins (pMHC) molecules per μm 2. We demonstrate that these substrates induce T cell responses in a pMHC dose-dependent manner and that the number of presented pMHCs dominates over local pMHC density.

AB - Antigen recognition is a key event during T cell activation. Here, we introduce nanopatterned antigen arrays that mimic the antigen presenting cell surface during T cell activation. The assessment of activation related events revealed the requirement of a minimal density of 90-140 stimulating major histocompatibility complex class II proteins (pMHC) molecules per μm 2. We demonstrate that these substrates induce T cell responses in a pMHC dose-dependent manner and that the number of presented pMHCs dominates over local pMHC density.

KW - Nanopattern

KW - T cell activation

KW - TCR microclusters

KW - artificial antigen-presenting cell

KW - immunological synapse

KW - nanostructures

KW - Humans

KW - Synapses/immunology

KW - Major Histocompatibility Complex/immunology

KW - Membrane Proteins/immunology

KW - Lymphocyte Activation/immunology

KW - Nanoparticles/chemistry

KW - Antigen-Presenting Cells/chemistry

KW - T-Lymphocytes/chemistry

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U2 - 10.1021/nl403266t

DO - 10.1021/nl403266t

M3 - Article

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SN - 1530-6984

VL - 13

SP - 5619

EP - 5626

JO - Nano Letters

JF - Nano Letters

IS - 11

ER -

T cell activation is determined by the number of presented antigens.

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Keywords

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