Sustainable extraction of valuable components from Spirulina assisted by pulsed electric fields technology

Anna Käferböck; Sergiy Smetana; Ronald de Vos; Christoph Schwarz; Stefan Toepfl; Oleksii Parniakov

doi:10.1016/j.algal.2020.101914

Sustainable extraction of valuable components from Spirulina assisted by pulsed electric fields technology

Anna Käferböck, Sergiy Smetana, Ronald de Vos, Christoph Schwarz, Stefan Toepfl, Oleksii Parniakov

Research Center Wels

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75 Citations (Scopus)

Abstract

The study investigated the potential of pulsed electric fields (PEF) technology application for the improvement of cell disintegration and subsequent extraction/fractionation of cyanobacteria Arthrospira maxima into valuable components (phycocyanin, proteins, oils and carbohydrates). Pulsed electric fields were applied to fresh A. maxima suspension, dried powder and dried sticks in combination with water as extraction solvent and freeze-thawing. Pulsed electric fields application for cell disruption reached approximately 90% increase of C-phycocyanin extraction compared to bead milling. Obtained fractions of phycocyanin and bulk proteins were also of higher purity and had twice lower environmental impact than similar fractions obtained without Pulsed electric fields treatment. Extracts with improved purity can be directly applied in pharma and food industry without any further processing and purification steps.

Original language	English
Article number	101914
Journal	Algal Research
Volume	48
DOIs	https://doi.org/10.1016/j.algal.2020.101914
Publication status	Published - Jun 2020

Keywords

Arthrospira maxima, Spirulina, phycocyanin
Electroporation
PEF
Pulsed electric field

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10.1016/j.algal.2020.101914

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title = "Sustainable extraction of valuable components from Spirulina assisted by pulsed electric fields technology",

abstract = "The study investigated the potential of pulsed electric fields (PEF) technology application for the improvement of cell disintegration and subsequent extraction/fractionation of cyanobacteria Arthrospira maxima into valuable components (phycocyanin, proteins, oils and carbohydrates). Pulsed electric fields were applied to fresh A. maxima suspension, dried powder and dried sticks in combination with water as extraction solvent and freeze-thawing. Pulsed electric fields application for cell disruption reached approximately 90% increase of C-phycocyanin extraction compared to bead milling. Obtained fractions of phycocyanin and bulk proteins were also of higher purity and had twice lower environmental impact than similar fractions obtained without Pulsed electric fields treatment. Extracts with improved purity can be directly applied in pharma and food industry without any further processing and purification steps.",

keywords = "Arthrospira maxima, Spirulina, phycocyanin, Electroporation, PEF, Pulsed electric field",

author = "Anna K{\"a}ferb{\"o}ck and Sergiy Smetana and {de Vos}, Ronald and Christoph Schwarz and Stefan Toepfl and Oleksii Parniakov",

note = "Funding Information: The work was funded as a part of eAlgae.2 project (project number 2-4.2): “Treatment of algae with electric pulsed fields (PEF)” in a framework of FOOD2020 Phase II program of Interreg V between Germany and the Netherlands.The work was co-financed by a FOOD2020 — Zukunftsoffensive Lebensmittelwirtschaft as a part of eAlgae.2 project. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = jun,

doi = "10.1016/j.algal.2020.101914",

language = "English",

volume = "48",

journal = "Algal Research",

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AU - Käferböck, Anna

AU - Smetana, Sergiy

AU - de Vos, Ronald

AU - Schwarz, Christoph

AU - Toepfl, Stefan

AU - Parniakov, Oleksii

N1 - Funding Information: The work was funded as a part of eAlgae.2 project (project number 2-4.2): “Treatment of algae with electric pulsed fields (PEF)” in a framework of FOOD2020 Phase II program of Interreg V between Germany and the Netherlands.The work was co-financed by a FOOD2020 — Zukunftsoffensive Lebensmittelwirtschaft as a part of eAlgae.2 project. Publisher Copyright: © 2020 Elsevier B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/6

Y1 - 2020/6

N2 - The study investigated the potential of pulsed electric fields (PEF) technology application for the improvement of cell disintegration and subsequent extraction/fractionation of cyanobacteria Arthrospira maxima into valuable components (phycocyanin, proteins, oils and carbohydrates). Pulsed electric fields were applied to fresh A. maxima suspension, dried powder and dried sticks in combination with water as extraction solvent and freeze-thawing. Pulsed electric fields application for cell disruption reached approximately 90% increase of C-phycocyanin extraction compared to bead milling. Obtained fractions of phycocyanin and bulk proteins were also of higher purity and had twice lower environmental impact than similar fractions obtained without Pulsed electric fields treatment. Extracts with improved purity can be directly applied in pharma and food industry without any further processing and purification steps.

AB - The study investigated the potential of pulsed electric fields (PEF) technology application for the improvement of cell disintegration and subsequent extraction/fractionation of cyanobacteria Arthrospira maxima into valuable components (phycocyanin, proteins, oils and carbohydrates). Pulsed electric fields were applied to fresh A. maxima suspension, dried powder and dried sticks in combination with water as extraction solvent and freeze-thawing. Pulsed electric fields application for cell disruption reached approximately 90% increase of C-phycocyanin extraction compared to bead milling. Obtained fractions of phycocyanin and bulk proteins were also of higher purity and had twice lower environmental impact than similar fractions obtained without Pulsed electric fields treatment. Extracts with improved purity can be directly applied in pharma and food industry without any further processing and purification steps.

KW - Arthrospira maxima, Spirulina, phycocyanin

KW - Electroporation

KW - PEF

KW - Pulsed electric field

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DO - 10.1016/j.algal.2020.101914

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JF - Algal Research

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Sustainable extraction of valuable components from Spirulina assisted by pulsed electric fields technology

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Keywords

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