Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells

Badri Aekbote, Tamas Fekete, Jaroslaw Jacak, Gaszton Vizsnyiczai, P Ormos, Lorand Kelemen

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

We introduce a method that combines two-photon polymerization (TPP) and surface functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures were coated specifically with a multilayer of the protein streptavidin and nonspecifically with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein density on their surfaces was quantified for various coating methods. The streptavidin-coated structures were shown to attach to biotinated cells reproducibly. We performed basic indirect optical micromanipulation tasks with attached structure-cell couples using complex structures and a multi-focus optical trap. The use of such extended manipulators for indirect optical trapping ensures to keep a safe distance between the trapping beams and the sensitive cell and enables their 6 degrees of freedom actuation.

Original languageEnglish
Pages (from-to)45-56
Number of pages12
JournalBiomedical Optics Express
Volume7
Issue number1
DOIs
Publication statusPublished - 7 Dec 2015

Keywords

  • Biomaterials
  • Fluorescence microscopy
  • Laser materials processing
  • Microstructure fabrication
  • Optical tweezers or optical manipulation
  • Polymers
  • Spatial light modulators
  • Thin films
  • Ultrafast lasers

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