Superresolution fluorescence microscopy using saturated modulation quenching (SMoQ)

Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Thomas A. Klar, Thomas Berer

Research output: Chapter in Book/Report/Conference proceedingsConference contributionpeer-review

1 Citation (Scopus)

Abstract

In this work, we demonstrate a new technique which has the potential for super-resolution fluorescence imaging. In this technique, similar to STED microscopy, a tightly focused intensity-modulated excitation beam and a donut shaped cw beam are confocally raster-scanned over the sample. In contrast to STED microscopy, both beams need to be absorbed by the fluorophore. A lock-in amplifier is used to measure only the modulated fluorescence. Sufficiently high cw donut beam intensities lead to saturation of the fluorophores in the outer rim of the modulated point spread function which enables resolution enhancement. Theoretically, sub-diffraction resolution can be achieved.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging XII
EditorsFelix Koberling, Zygmunt K. Gryczynski, Ingo Gregor
PublisherSPIE
ISBN (Electronic)9781510624108
DOIs
Publication statusPublished - 2019
EventSingle Molecule Spectroscopy and Superresolution Imaging XII 2019 - San Francisco, United States
Duration: 2 Feb 20193 Feb 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10884
ISSN (Print)1605-7422

Conference

ConferenceSingle Molecule Spectroscopy and Superresolution Imaging XII 2019
CountryUnited States
CitySan Francisco
Period02.02.201903.02.2019

Keywords

  • Fluorescence microscopy
  • Intensity modulation
  • Saturated modulation quenching
  • Super-resolution microscopy

Fingerprint Dive into the research topics of 'Superresolution fluorescence microscopy using saturated modulation quenching (SMoQ)'. Together they form a unique fingerprint.

Cite this