STED controlled photobleaching for sub-diffractional optical nanopatterning

Eljesa Murtezi, Sujitha Puthukodan, Bianca Buchegger, Jaroslaw Jacak, Thomas A. Klar

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Laser-assisted protein adsorption by photobleaching (LAPAP) is a versatile tool to nanopattern proteins on the micrometer scale. Sub-micron patterning is, however, difficult due to diffraction. We show that, similar to stimulated emission depletion (STED) microscopy, a depleting beam can effectively suppress LAPAP and hence is apt to locally control LAPAP in order to write sub-diffractional lines of proteins. Specifically, we attach biotinylated Atto 390 to glass substrates and incubate with Alexa 555 labeled streptavidin. The Alexa 555 is subsequently imaged with STED nanoscopy. The method is currently limited by diffusion of the biotinylated Atto 390 molecules.

Original languageEnglish
Article number044003
JournalJPhys Photonics
Issue number4
Publication statusPublished - 24 Sept 2020


  • Light induced protein patterning
  • Localized photochemistry
  • STED inspired lithography
  • Stimulated emission depletion (STED)


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