STED controlled photobleaching for sub-diffractional optical nanopatterning

Eljesa Murtezi; Sujitha Puthukodan; Bianca Buchegger; Jaroslaw Jacak; Thomas A. Klar

doi:10.1088/2515-7647/abb219

STED controlled photobleaching for sub-diffractional optical nanopatterning

Eljesa Murtezi, Sujitha Puthukodan, Bianca Buchegger, Jaroslaw Jacak, Thomas A. Klar^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Laser-assisted protein adsorption by photobleaching (LAPAP) is a versatile tool to nanopattern proteins on the micrometer scale. Sub-micron patterning is, however, difficult due to diffraction. We show that, similar to stimulated emission depletion (STED) microscopy, a depleting beam can effectively suppress LAPAP and hence is apt to locally control LAPAP in order to write sub-diffractional lines of proteins. Specifically, we attach biotinylated Atto 390 to glass substrates and incubate with Alexa 555 labeled streptavidin. The Alexa 555 is subsequently imaged with STED nanoscopy. The method is currently limited by diffusion of the biotinylated Atto 390 molecules.

Original language	English
Article number	044003
Journal	JPhys Photonics
Volume	2
Issue number	4
DOIs	https://doi.org/10.1088/2515-7647/abb219
Publication status	Published - 24 Sept 2020

Keywords

Light induced protein patterning
Localized photochemistry
STED inspired lithography
Stimulated emission depletion (STED)

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10.1088/2515-7647/abb219

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title = "STED controlled photobleaching for sub-diffractional optical nanopatterning",

abstract = "Laser-assisted protein adsorption by photobleaching (LAPAP) is a versatile tool to nanopattern proteins on the micrometer scale. Sub-micron patterning is, however, difficult due to diffraction. We show that, similar to stimulated emission depletion (STED) microscopy, a depleting beam can effectively suppress LAPAP and hence is apt to locally control LAPAP in order to write sub-diffractional lines of proteins. Specifically, we attach biotinylated Atto 390 to glass substrates and incubate with Alexa 555 labeled streptavidin. The Alexa 555 is subsequently imaged with STED nanoscopy. The method is currently limited by diffusion of the biotinylated Atto 390 molecules. ",

keywords = "Light induced protein patterning, Localized photochemistry, STED inspired lithography, Stimulated emission depletion (STED)",

author = "Eljesa Murtezi and Sujitha Puthukodan and Bianca Buchegger and Jaroslaw Jacak and Klar, {Thomas A.}",

note = "Funding Information: We would like to thank Bernhard Fragner, Alfred Nimmervoll and Heidi Piglmayer-Brezina for technical support. This work was supported by the Doctorate College program {\textquoteleft}Nano-Analytics of Cellular Systems (NanoCell){\textquoteright} of the Austrian Science Fund (FWF) (Grant Number W 1250) and by the State of Upper Austria. Publisher Copyright: {\textcopyright} 2020 The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

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doi = "10.1088/2515-7647/abb219",

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AU - Murtezi, Eljesa

AU - Puthukodan, Sujitha

AU - Buchegger, Bianca

AU - Jacak, Jaroslaw

AU - Klar, Thomas A.

N1 - Funding Information: We would like to thank Bernhard Fragner, Alfred Nimmervoll and Heidi Piglmayer-Brezina for technical support. This work was supported by the Doctorate College program ‘Nano-Analytics of Cellular Systems (NanoCell)’ of the Austrian Science Fund (FWF) (Grant Number W 1250) and by the State of Upper Austria. Publisher Copyright: © 2020 The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2020/9/24

Y1 - 2020/9/24

N2 - Laser-assisted protein adsorption by photobleaching (LAPAP) is a versatile tool to nanopattern proteins on the micrometer scale. Sub-micron patterning is, however, difficult due to diffraction. We show that, similar to stimulated emission depletion (STED) microscopy, a depleting beam can effectively suppress LAPAP and hence is apt to locally control LAPAP in order to write sub-diffractional lines of proteins. Specifically, we attach biotinylated Atto 390 to glass substrates and incubate with Alexa 555 labeled streptavidin. The Alexa 555 is subsequently imaged with STED nanoscopy. The method is currently limited by diffusion of the biotinylated Atto 390 molecules.

AB - Laser-assisted protein adsorption by photobleaching (LAPAP) is a versatile tool to nanopattern proteins on the micrometer scale. Sub-micron patterning is, however, difficult due to diffraction. We show that, similar to stimulated emission depletion (STED) microscopy, a depleting beam can effectively suppress LAPAP and hence is apt to locally control LAPAP in order to write sub-diffractional lines of proteins. Specifically, we attach biotinylated Atto 390 to glass substrates and incubate with Alexa 555 labeled streptavidin. The Alexa 555 is subsequently imaged with STED nanoscopy. The method is currently limited by diffusion of the biotinylated Atto 390 molecules.

KW - Light induced protein patterning

KW - Localized photochemistry

KW - STED inspired lithography

KW - Stimulated emission depletion (STED)

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DO - 10.1088/2515-7647/abb219

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JO - JPhys Photonics

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M1 - 044003

ER -

STED controlled photobleaching for sub-diffractional optical nanopatterning

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Keywords

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