STED controlled photobleaching for sub-diffractional optical nanopatterning

Eljesa Murtezi, Sujitha Puthukodan, Bianca Buchegger, Jaroslaw Jacak, Thomas A. Klar

Research output: Contribution to journalArticlepeer-review

Abstract

Laser-assisted protein adsorption by photobleaching (LAPAP) is a versatile tool to nanopattern proteins on the micrometer scale. Sub-micron patterning is, however, difficult due to diffraction. We show that, similar to stimulated emission depletion (STED) microscopy, a depleting beam can effectively suppress LAPAP and hence is apt to locally control LAPAP in order to write sub-diffractional lines of proteins. Specifically, we attach biotinylated Atto 390 to glass substrates and incubate with Alexa 555 labeled streptavidin. The Alexa 555 is subsequently imaged with STED nanoscopy. The method is currently limited by diffusion of the biotinylated Atto 390 molecules.

Original languageEnglish
Article number044003
JournalJPhys Photonics
Volume2
Issue number4
DOIs
Publication statusPublished - 24 Sep 2020

Keywords

  • Light induced protein patterning
  • Localized photochemistry
  • STED inspired lithography
  • Stimulated emission depletion (STED)

Fingerprint Dive into the research topics of 'STED controlled photobleaching for sub-diffractional optical nanopatterning'. Together they form a unique fingerprint.

Cite this