TY - JOUR
T1 - STED controlled photobleaching for sub-diffractional optical nanopatterning
AU - Murtezi, Eljesa
AU - Puthukodan, Sujitha
AU - Buchegger, Bianca
AU - Jacak, Jaroslaw
AU - Klar, Thomas A.
N1 - Funding Information:
We would like to thank Bernhard Fragner, Alfred Nimmervoll and Heidi Piglmayer-Brezina for technical support. This work was supported by the Doctorate College program ‘Nano-Analytics of Cellular Systems (NanoCell)’ of the Austrian Science Fund (FWF) (Grant Number W 1250) and by the State of Upper Austria.
Publisher Copyright:
© 2020 The Author(s).
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2020/9/24
Y1 - 2020/9/24
N2 - Laser-assisted protein adsorption by photobleaching (LAPAP) is a versatile tool to nanopattern proteins on the micrometer scale. Sub-micron patterning is, however, difficult due to diffraction. We show that, similar to stimulated emission depletion (STED) microscopy, a depleting beam can effectively suppress LAPAP and hence is apt to locally control LAPAP in order to write sub-diffractional lines of proteins. Specifically, we attach biotinylated Atto 390 to glass substrates and incubate with Alexa 555 labeled streptavidin. The Alexa 555 is subsequently imaged with STED nanoscopy. The method is currently limited by diffusion of the biotinylated Atto 390 molecules.
AB - Laser-assisted protein adsorption by photobleaching (LAPAP) is a versatile tool to nanopattern proteins on the micrometer scale. Sub-micron patterning is, however, difficult due to diffraction. We show that, similar to stimulated emission depletion (STED) microscopy, a depleting beam can effectively suppress LAPAP and hence is apt to locally control LAPAP in order to write sub-diffractional lines of proteins. Specifically, we attach biotinylated Atto 390 to glass substrates and incubate with Alexa 555 labeled streptavidin. The Alexa 555 is subsequently imaged with STED nanoscopy. The method is currently limited by diffusion of the biotinylated Atto 390 molecules.
KW - Light induced protein patterning
KW - Localized photochemistry
KW - STED inspired lithography
KW - Stimulated emission depletion (STED)
UR - http://www.scopus.com/inward/record.url?scp=85092510390&partnerID=8YFLogxK
U2 - 10.1088/2515-7647/abb219
DO - 10.1088/2515-7647/abb219
M3 - Article
AN - SCOPUS:85092510390
VL - 2
JO - JPhys Photonics
JF - JPhys Photonics
SN - 2515-7647
IS - 4
M1 - 044003
ER -