Single molecule reader for high-throughput bioanalysis

Jan Hesse; Jaroslaw Jacak; Alois Sonnleitner; Otmar Höglinger; Gerhard Schütz

doi:10.1021/ac049300f

Single molecule reader for high-throughput bioanalysis

Jan Hesse, Jaroslaw Jacak, Alois Sonnleitner, Otmar Höglinger, Gerhard Schütz

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52 Citations (Scopus)

Abstract

We report here the development of a device for single-molecule imaging on large surface areas. A CCD camera operated in time delay and integration mode is synchronized with the movement of a sample scanning stage, enabling continuous data acquisition. Experiments on single fluorescent lipid molecules in supported lipid bilayers and on stained living cells demonstrate the capabilities of the method. Areas of up to 5 x 5 mm² were recorded within 11 min at a pixel size of 129 nm.

Original language	English
Pages (from-to)	5960-5964
Number of pages	5
Journal	Analytical Chemistry
Volume	76
Issue number	19
DOIs	https://doi.org/10.1021/ac049300f
Publication status	Published - 1 Oct 2004

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title = "Single molecule reader for high-throughput bioanalysis",

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AU - Jacak, Jaroslaw

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AU - Höglinger, Otmar

AU - Schütz, Gerhard

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Single molecule reader for high-throughput bioanalysis

Abstract

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