Serum and Lipoprotein Particle miRNA Profile in Uremia Patients

Markus Axmann; Sabine M. Meier; Andreas Karner; Witta Strobl; Herbert Stangl; Birgit Plochberger

doi:10.3390/genes9110533

Serum and Lipoprotein Particle miRNA Profile in Uremia Patients

Markus Axmann, Sabine M. Meier, Andreas Karner, Witta Strobl, Herbert Stangl, Birgit Plochberger

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

microRNAs (miRNAs) are post-transcriptional regulators of messenger RNA (mRNA), and transported through the whole organism by—but not limited to—lipoprotein particles. Here, we address the miRNA profile in serum and lipoprotein particles of healthy individuals in comparison with patients with uremia. Moreover, we quantitatively determined the cellular lipoprotein-particle-uptake dependence on the density of lipoprotein particle receptors and present a method for enhancement of the transfer efficiency. We observed a significant increase of the cellular miRNA level using reconstituted high-density lipoprotein (HDL) particles artificially loaded with miRNA, whereas incubation with native HDL particles yielded no measurable effect. Thus, we conclude that no relevant effect of lipoprotein-particle-mediated miRNA-transfer exists under in vivo conditions though the miRNA profile of lipoprotein particles can be used as a diagnostic marker.

Original language	English
Article number	533
Number of pages	17
Journal	Genes
Volume	9
Issue number	11
DOIs	https://doi.org/10.3390/genes9110533
Publication status	Published - 5 Nov 2018

Keywords

Lipoprotein
MicroRNA
Transfer
Uremia

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10.3390/genes9110533

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title = "Serum and Lipoprotein Particle miRNA Profile in Uremia Patients",

abstract = "microRNAs (miRNAs) are post-transcriptional regulators of messenger RNA (mRNA), and transported through the whole organism by—but not limited to—lipoprotein particles. Here, we address the miRNA profile in serum and lipoprotein particles of healthy individuals in comparison with patients with uremia. Moreover, we quantitatively determined the cellular lipoprotein-particle-uptake dependence on the density of lipoprotein particle receptors and present a method for enhancement of the transfer efficiency. We observed a significant increase of the cellular miRNA level using reconstituted high-density lipoprotein (HDL) particles artificially loaded with miRNA, whereas incubation with native HDL particles yielded no measurable effect. Thus, we conclude that no relevant effect of lipoprotein-particle-mediated miRNA-transfer exists under in vivo conditions though the miRNA profile of lipoprotein particles can be used as a diagnostic marker.",

keywords = "Lipoprotein, MicroRNA, Transfer, Uremia",

author = "Markus Axmann and Meier, {Sabine M.} and Andreas Karner and Witta Strobl and Herbert Stangl and Birgit Plochberger",

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TY - JOUR

T1 - Serum and Lipoprotein Particle miRNA Profile in Uremia Patients

AU - Axmann, Markus

AU - Meier, Sabine M.

AU - Karner, Andreas

AU - Strobl, Witta

AU - Stangl, Herbert

AU - Plochberger, Birgit

PY - 2018/11/5

Y1 - 2018/11/5

N2 - microRNAs (miRNAs) are post-transcriptional regulators of messenger RNA (mRNA), and transported through the whole organism by—but not limited to—lipoprotein particles. Here, we address the miRNA profile in serum and lipoprotein particles of healthy individuals in comparison with patients with uremia. Moreover, we quantitatively determined the cellular lipoprotein-particle-uptake dependence on the density of lipoprotein particle receptors and present a method for enhancement of the transfer efficiency. We observed a significant increase of the cellular miRNA level using reconstituted high-density lipoprotein (HDL) particles artificially loaded with miRNA, whereas incubation with native HDL particles yielded no measurable effect. Thus, we conclude that no relevant effect of lipoprotein-particle-mediated miRNA-transfer exists under in vivo conditions though the miRNA profile of lipoprotein particles can be used as a diagnostic marker.

AB - microRNAs (miRNAs) are post-transcriptional regulators of messenger RNA (mRNA), and transported through the whole organism by—but not limited to—lipoprotein particles. Here, we address the miRNA profile in serum and lipoprotein particles of healthy individuals in comparison with patients with uremia. Moreover, we quantitatively determined the cellular lipoprotein-particle-uptake dependence on the density of lipoprotein particle receptors and present a method for enhancement of the transfer efficiency. We observed a significant increase of the cellular miRNA level using reconstituted high-density lipoprotein (HDL) particles artificially loaded with miRNA, whereas incubation with native HDL particles yielded no measurable effect. Thus, we conclude that no relevant effect of lipoprotein-particle-mediated miRNA-transfer exists under in vivo conditions though the miRNA profile of lipoprotein particles can be used as a diagnostic marker.

KW - Lipoprotein

KW - MicroRNA

KW - Transfer

KW - Uremia

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U2 - 10.3390/genes9110533

DO - 10.3390/genes9110533

M3 - Article

SN - 2073-4425

VL - 9

JO - Genes

JF - Genes

IS - 11

M1 - 533

ER -

Serum and Lipoprotein Particle miRNA Profile in Uremia Patients

Abstract

Keywords

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