Second Harmonic Atomic Force Microscopy Imaging of Live and Fixed Mammalian Cells

Alexander Dulebo; Johannes Preiner; Ferry Kienberger; Gerald Kada; Christian Rankl; Lilia Chtcheglova; Constanze Lamprecht; David Kaftan; Peter Hinterdorfer

doi:10.1016/j.ultramic.2009.03.020

Second Harmonic Atomic Force Microscopy Imaging of Live and Fixed Mammalian Cells

Alexander Dulebo
, Johannes Preiner
, Ferry Kienberger
, Gerald Kada
, Christian Rankl
, Lilia Chtcheglova
, Constanze Lamprecht
, David Kaftan
, Peter Hinterdorfer

Research output: Contribution to journal › Article

25 Citations (Scopus)

Abstract

Higherharmoniccontributionsinthemovementofanoscillatingatomicforcemicroscopy(AFM) cantileveraregeneratedbynonlineartip–sampleinteractions,yieldingadditionalinformationonstructure andphysicalpropertiessuchassamplestiffness.Higherharmonicamplitudesarestronglyenhancedin liquidcomparedtotheoperationinair,andwerepreviouslyreportedtoresultinbetterstructural resolutioninhighlyorganizedlatticesofproteinsinbacterialS-layersandviralcapsids[J.Preiner,J.Tang, V. Pastushenko,P.Hinterdorfer,Phys.Rev.Lett.99(2007)046102].Wecomparedfirstandsecond harmonicsAFMimagingofliveandfixedhumanlungepithelialcells,andmicrovascularendothelialcells frommousemyocardium(MyEnd).Phase–distancecyclesrevealedthatthesecondharmonicphaseis8 timesmoresensitivethanthefirstharmonicphasewithrespecttovariationsinthedistancebetween cantileverandsamplesurface.Frequencyspectrawereacquiredatdifferentpositionsonlivingandfixed cellswithsecondharmonicamplitudevaluescorrelatingwiththesamplestiffness.Weconcludethat variationsinsamplestiffnessandcorrespondingchangesinthecantilever–sampledistance,lattereffect causedbythefinitefeedbackresponse,resultinsecondharmonicimageswithimprovedcontrastand informationthatisnotattainableinthefundamentalfrequencyofanoscillatingcantilever.

Original language	English
Pages (from-to)	1056-1060
Number of pages	5
Journal	Ultramicroscopy
Volume	109
Issue number	8
DOIs	https://doi.org/10.1016/j.ultramic.2009.03.020
Publication status	Published - Jul 2009

Keywords

Atomic force microscopy
Cells
Higher harmonic
Live cell imaging

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10.1016/j.ultramic.2009.03.020

Cite this

@article{f27c6f926ef24967801a24a04469eac2,

title = "Second Harmonic Atomic Force Microscopy Imaging of Live and Fixed Mammalian Cells",

abstract = "Higherharmoniccontributionsinthemovementofanoscillatingatomicforcemicroscopy(AFM) cantileveraregeneratedbynonlineartip–sampleinteractions,yieldingadditionalinformationonstructure andphysicalpropertiessuchassamplestiffness.Higherharmonicamplitudesarestronglyenhancedin liquidcomparedtotheoperationinair,andwerepreviouslyreportedtoresultinbetterstructural resolutioninhighlyorganizedlatticesofproteinsinbacterialS-layersandviralcapsids[J.Preiner,J.Tang, V. Pastushenko,P.Hinterdorfer,Phys.Rev.Lett.99(2007)046102].Wecomparedfirstandsecond harmonicsAFMimagingofliveandfixedhumanlungepithelialcells,andmicrovascularendothelialcells frommousemyocardium(MyEnd).Phase–distancecyclesrevealedthatthesecondharmonicphaseis8 timesmoresensitivethanthefirstharmonicphasewithrespecttovariationsinthedistancebetween cantileverandsamplesurface.Frequencyspectrawereacquiredatdifferentpositionsonlivingandfixed cellswithsecondharmonicamplitudevaluescorrelatingwiththesamplestiffness.Weconcludethat variationsinsamplestiffnessandcorrespondingchangesinthecantilever–sampledistance,lattereffect causedbythefinitefeedbackresponse,resultinsecondharmonicimageswithimprovedcontrastand informationthatisnotattainableinthefundamentalfrequencyofanoscillatingcantilever.",

keywords = "Atomic force microscopy, Cells, Higher harmonic, Live cell imaging",

author = "Alexander Dulebo and Johannes Preiner and Ferry Kienberger and Gerald Kada and Christian Rankl and Lilia Chtcheglova and Constanze Lamprecht and David Kaftan and Peter Hinterdorfer",

note = "Funding Information: The present study was supported by grants from the Swedish Cancer Society, the Medical Faculty, UmeI University, and Lions Research Foundation, Umeb, Sweden.",

year = "2009",

month = jul,

doi = "10.1016/j.ultramic.2009.03.020",

language = "English",

volume = "109",

pages = "1056--1060",

journal = "Ultramicroscopy",

issn = "1879-2723",

publisher = "Elsevier",

number = "8",

}

TY - JOUR

T1 - Second Harmonic Atomic Force Microscopy Imaging of Live and Fixed Mammalian Cells

AU - Dulebo, Alexander

AU - Preiner, Johannes

AU - Kienberger, Ferry

AU - Kada, Gerald

AU - Rankl, Christian

AU - Chtcheglova, Lilia

AU - Lamprecht, Constanze

AU - Kaftan, David

AU - Hinterdorfer, Peter

N1 - Funding Information: The present study was supported by grants from the Swedish Cancer Society, the Medical Faculty, UmeI University, and Lions Research Foundation, Umeb, Sweden.

PY - 2009/7

Y1 - 2009/7

N2 - Higherharmoniccontributionsinthemovementofanoscillatingatomicforcemicroscopy(AFM) cantileveraregeneratedbynonlineartip–sampleinteractions,yieldingadditionalinformationonstructure andphysicalpropertiessuchassamplestiffness.Higherharmonicamplitudesarestronglyenhancedin liquidcomparedtotheoperationinair,andwerepreviouslyreportedtoresultinbetterstructural resolutioninhighlyorganizedlatticesofproteinsinbacterialS-layersandviralcapsids[J.Preiner,J.Tang, V. Pastushenko,P.Hinterdorfer,Phys.Rev.Lett.99(2007)046102].Wecomparedfirstandsecond harmonicsAFMimagingofliveandfixedhumanlungepithelialcells,andmicrovascularendothelialcells frommousemyocardium(MyEnd).Phase–distancecyclesrevealedthatthesecondharmonicphaseis8 timesmoresensitivethanthefirstharmonicphasewithrespecttovariationsinthedistancebetween cantileverandsamplesurface.Frequencyspectrawereacquiredatdifferentpositionsonlivingandfixed cellswithsecondharmonicamplitudevaluescorrelatingwiththesamplestiffness.Weconcludethat variationsinsamplestiffnessandcorrespondingchangesinthecantilever–sampledistance,lattereffect causedbythefinitefeedbackresponse,resultinsecondharmonicimageswithimprovedcontrastand informationthatisnotattainableinthefundamentalfrequencyofanoscillatingcantilever.

AB - Higherharmoniccontributionsinthemovementofanoscillatingatomicforcemicroscopy(AFM) cantileveraregeneratedbynonlineartip–sampleinteractions,yieldingadditionalinformationonstructure andphysicalpropertiessuchassamplestiffness.Higherharmonicamplitudesarestronglyenhancedin liquidcomparedtotheoperationinair,andwerepreviouslyreportedtoresultinbetterstructural resolutioninhighlyorganizedlatticesofproteinsinbacterialS-layersandviralcapsids[J.Preiner,J.Tang, V. Pastushenko,P.Hinterdorfer,Phys.Rev.Lett.99(2007)046102].Wecomparedfirstandsecond harmonicsAFMimagingofliveandfixedhumanlungepithelialcells,andmicrovascularendothelialcells frommousemyocardium(MyEnd).Phase–distancecyclesrevealedthatthesecondharmonicphaseis8 timesmoresensitivethanthefirstharmonicphasewithrespecttovariationsinthedistancebetween cantileverandsamplesurface.Frequencyspectrawereacquiredatdifferentpositionsonlivingandfixed cellswithsecondharmonicamplitudevaluescorrelatingwiththesamplestiffness.Weconcludethat variationsinsamplestiffnessandcorrespondingchangesinthecantilever–sampledistance,lattereffect causedbythefinitefeedbackresponse,resultinsecondharmonicimageswithimprovedcontrastand informationthatisnotattainableinthefundamentalfrequencyofanoscillatingcantilever.

KW - Atomic force microscopy

KW - Cells

KW - Higher harmonic

KW - Live cell imaging

UR - https://www.scopus.com/pages/publications/67449146904

U2 - 10.1016/j.ultramic.2009.03.020

DO - 10.1016/j.ultramic.2009.03.020

M3 - Article

SN - 1879-2723

VL - 109

SP - 1056

EP - 1060

JO - Ultramicroscopy

JF - Ultramicroscopy

IS - 8

ER -

Second Harmonic Atomic Force Microscopy Imaging of Live and Fixed Mammalian Cells

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Keywords

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