Second Harmonic Atomic Force Microscopy Imaging of Live and Fixed Mammalian Cells

Alexander Dulebo; Johannes Preiner; Ferry Kienberger; Gerald Kada; Christian Rankl; Lilia Chtcheglova; Constanze Lamprecht; David Kaftan; Peter Hinterdorfer

doi:10.1016/j.ultramic.2009.03.020

Second Harmonic Atomic Force Microscopy Imaging of Live and Fixed Mammalian Cells

Alexander Dulebo, Johannes Preiner, Ferry Kienberger, Gerald Kada, Christian Rankl, Lilia Chtcheglova, Constanze Lamprecht, David Kaftan, Peter Hinterdorfer

Research output: Contribution to journal › Article

25 Citations (Scopus)

Abstract

Higherharmoniccontributionsinthemovementofanoscillatingatomicforcemicroscopy(AFM) cantileveraregeneratedbynonlineartip–sampleinteractions,yieldingadditionalinformationonstructure andphysicalpropertiessuchassamplestiffness.Higherharmonicamplitudesarestronglyenhancedin liquidcomparedtotheoperationinair,andwerepreviouslyreportedtoresultinbetterstructural resolutioninhighlyorganizedlatticesofproteinsinbacterialS-layersandviralcapsids[J.Preiner,J.Tang, V. Pastushenko,P.Hinterdorfer,Phys.Rev.Lett.99(2007)046102].Wecomparedfirstandsecond harmonicsAFMimagingofliveandfixedhumanlungepithelialcells,andmicrovascularendothelialcells frommousemyocardium(MyEnd).Phase–distancecyclesrevealedthatthesecondharmonicphaseis8 timesmoresensitivethanthefirstharmonicphasewithrespecttovariationsinthedistancebetween cantileverandsamplesurface.Frequencyspectrawereacquiredatdifferentpositionsonlivingandfixed cellswithsecondharmonicamplitudevaluescorrelatingwiththesamplestiffness.Weconcludethat variationsinsamplestiffnessandcorrespondingchangesinthecantilever–sampledistance,lattereffect causedbythefinitefeedbackresponse,resultinsecondharmonicimageswithimprovedcontrastand informationthatisnotattainableinthefundamentalfrequencyofanoscillatingcantilever.

Original language	English
Pages (from-to)	1056-1060
Number of pages	5
Journal	Ultramicroscopy
Volume	109
Issue number	8
DOIs	https://doi.org/10.1016/j.ultramic.2009.03.020
Publication status	Published - Jul 2009

Keywords

Atomic force microscopy
Cells
Higher harmonic
Live cell imaging

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10.1016/j.ultramic.2009.03.020

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@article{f27c6f926ef24967801a24a04469eac2,

title = "Second Harmonic Atomic Force Microscopy Imaging of Live and Fixed Mammalian Cells",

abstract = "Higherharmoniccontributionsinthemovementofanoscillatingatomicforcemicroscopy(AFM) cantileveraregeneratedbynonlineartip–sampleinteractions,yieldingadditionalinformationonstructure andphysicalpropertiessuchassamplestiffness.Higherharmonicamplitudesarestronglyenhancedin liquidcomparedtotheoperationinair,andwerepreviouslyreportedtoresultinbetterstructural resolutioninhighlyorganizedlatticesofproteinsinbacterialS-layersandviralcapsids[J.Preiner,J.Tang, V. Pastushenko,P.Hinterdorfer,Phys.Rev.Lett.99(2007)046102].Wecomparedfirstandsecond harmonicsAFMimagingofliveandfixedhumanlungepithelialcells,andmicrovascularendothelialcells frommousemyocardium(MyEnd).Phase–distancecyclesrevealedthatthesecondharmonicphaseis8 timesmoresensitivethanthefirstharmonicphasewithrespecttovariationsinthedistancebetween cantileverandsamplesurface.Frequencyspectrawereacquiredatdifferentpositionsonlivingandfixed cellswithsecondharmonicamplitudevaluescorrelatingwiththesamplestiffness.Weconcludethat variationsinsamplestiffnessandcorrespondingchangesinthecantilever–sampledistance,lattereffect causedbythefinitefeedbackresponse,resultinsecondharmonicimageswithimprovedcontrastand informationthatisnotattainableinthefundamentalfrequencyofanoscillatingcantilever.",

keywords = "Atomic force microscopy, Cells, Higher harmonic, Live cell imaging",

author = "Alexander Dulebo and Johannes Preiner and Ferry Kienberger and Gerald Kada and Christian Rankl and Lilia Chtcheglova and Constanze Lamprecht and David Kaftan and Peter Hinterdorfer",

note = "Funding Information: The present study was supported by grants from the Swedish Cancer Society, the Medical Faculty, UmeI University, and Lions Research Foundation, Umeb, Sweden.",

year = "2009",

month = jul,

doi = "10.1016/j.ultramic.2009.03.020",

language = "English",

volume = "109",

pages = "1056--1060",

journal = "Ultramicroscopy",

issn = "1879-2723",

publisher = "Elsevier",

number = "8",

}

TY - JOUR

T1 - Second Harmonic Atomic Force Microscopy Imaging of Live and Fixed Mammalian Cells

AU - Dulebo, Alexander

AU - Preiner, Johannes

AU - Kienberger, Ferry

AU - Kada, Gerald

AU - Rankl, Christian

AU - Chtcheglova, Lilia

AU - Lamprecht, Constanze

AU - Kaftan, David

AU - Hinterdorfer, Peter

N1 - Funding Information: The present study was supported by grants from the Swedish Cancer Society, the Medical Faculty, UmeI University, and Lions Research Foundation, Umeb, Sweden.

PY - 2009/7

Y1 - 2009/7

N2 - Higherharmoniccontributionsinthemovementofanoscillatingatomicforcemicroscopy(AFM) cantileveraregeneratedbynonlineartip–sampleinteractions,yieldingadditionalinformationonstructure andphysicalpropertiessuchassamplestiffness.Higherharmonicamplitudesarestronglyenhancedin liquidcomparedtotheoperationinair,andwerepreviouslyreportedtoresultinbetterstructural resolutioninhighlyorganizedlatticesofproteinsinbacterialS-layersandviralcapsids[J.Preiner,J.Tang, V. Pastushenko,P.Hinterdorfer,Phys.Rev.Lett.99(2007)046102].Wecomparedfirstandsecond harmonicsAFMimagingofliveandfixedhumanlungepithelialcells,andmicrovascularendothelialcells frommousemyocardium(MyEnd).Phase–distancecyclesrevealedthatthesecondharmonicphaseis8 timesmoresensitivethanthefirstharmonicphasewithrespecttovariationsinthedistancebetween cantileverandsamplesurface.Frequencyspectrawereacquiredatdifferentpositionsonlivingandfixed cellswithsecondharmonicamplitudevaluescorrelatingwiththesamplestiffness.Weconcludethat variationsinsamplestiffnessandcorrespondingchangesinthecantilever–sampledistance,lattereffect causedbythefinitefeedbackresponse,resultinsecondharmonicimageswithimprovedcontrastand informationthatisnotattainableinthefundamentalfrequencyofanoscillatingcantilever.

AB - Higherharmoniccontributionsinthemovementofanoscillatingatomicforcemicroscopy(AFM) cantileveraregeneratedbynonlineartip–sampleinteractions,yieldingadditionalinformationonstructure andphysicalpropertiessuchassamplestiffness.Higherharmonicamplitudesarestronglyenhancedin liquidcomparedtotheoperationinair,andwerepreviouslyreportedtoresultinbetterstructural resolutioninhighlyorganizedlatticesofproteinsinbacterialS-layersandviralcapsids[J.Preiner,J.Tang, V. Pastushenko,P.Hinterdorfer,Phys.Rev.Lett.99(2007)046102].Wecomparedfirstandsecond harmonicsAFMimagingofliveandfixedhumanlungepithelialcells,andmicrovascularendothelialcells frommousemyocardium(MyEnd).Phase–distancecyclesrevealedthatthesecondharmonicphaseis8 timesmoresensitivethanthefirstharmonicphasewithrespecttovariationsinthedistancebetween cantileverandsamplesurface.Frequencyspectrawereacquiredatdifferentpositionsonlivingandfixed cellswithsecondharmonicamplitudevaluescorrelatingwiththesamplestiffness.Weconcludethat variationsinsamplestiffnessandcorrespondingchangesinthecantilever–sampledistance,lattereffect causedbythefinitefeedbackresponse,resultinsecondharmonicimageswithimprovedcontrastand informationthatisnotattainableinthefundamentalfrequencyofanoscillatingcantilever.

KW - Atomic force microscopy

KW - Cells

KW - Higher harmonic

KW - Live cell imaging

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U2 - 10.1016/j.ultramic.2009.03.020

DO - 10.1016/j.ultramic.2009.03.020

M3 - Article

SN - 1879-2723

VL - 109

SP - 1056

EP - 1060

JO - Ultramicroscopy

JF - Ultramicroscopy

IS - 8

ER -

Second Harmonic Atomic Force Microscopy Imaging of Live and Fixed Mammalian Cells

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Keywords

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