Real-time 3D single-molecule localization microscopy analysis using lookup tables

Research output: Contribution to journalArticlepeer-review

Abstract

Herein, we present a new algorithm for real-time analysis of 3D single molecule localization microscopy images with a small impact on fitting accuracy using lookup-tables with discrete xyz-positions. The algorithm realizes real-time visualization during acquisition. We demonstrate its performance on simulated and measured data. Additionally, combining real-time fitting with a feedback loop controlling the activation laser pulse keeps the number of emitters per image frame constant.
Original languageEnglish
Pages (from-to)4955-4968
Number of pages14
JournalBiomedical Optics Express
Volume12
Issue number8
DOIs
Publication statusPublished - 1 Aug 2021

Keywords

  • Super resolution microscopy
  • Single Molecule Localization Microscopy
  • Microscopy
  • 3D dSTORM
  • 3D Localization Microscopy
  • Cells
  • Bioinformatics
  • Fitting Algorithm
  • Lookup Table
  • Software
  • Platelets
  • Simulation

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