PySpot: A python based framework for the assessment of laser-modified 3D microstructures for Windows and Raspbian

Hannah Janout, Bianca Buchegger, Andreas Haghofer, Dominic Höglinger, Jaroslaw Jacak, Stephan Winkler, Armin Hochreiner

Research output: Chapter in Book/Report/Conference proceedingsConference contributionpeer-review

Abstract

Biocompatible 3D microstructures created with laser lithography and modified for optimal cell growth with laser grafting, can imitate the 3D structure cells naturally grow in. For the evaluation of the quality and success of those 3D microstructures, specialized software is required. Our software PySpot can load 2D and 3D images and analyze those through image segmentation, edge detection, and surface plots. Additionally, the creation and modification of regions of interest (ROI) allow for the quality evaluation of specific areas in an image by intensity analysis. 3D rendering allows for identifying complex geometrical properties. Furthermore, PySpot is executable on Windows as well as on Raspbian, which makes it flexible to use.

Original languageEnglish
Title of host publicationBIOIMAGING 2020 - 7th International Conference on Bioimaging, Proceedings; Part of 13th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2020
EditorsFilipe Soares, Ana Fred, Hugo Gamboa
PublisherSciTePress
Pages135-142
Number of pages8
Volume2
ISBN (Electronic)9789897583988
Publication statusPublished - 2020
Event7th International Conference on Bioimaging, BIOIMAGING 2020 - Part of 13th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2020 - Valletta, Malta
Duration: 24 Feb 202026 Feb 2020

Publication series

NameBIOIMAGING 2020 - 7th International Conference on Bioimaging, Proceedings; Part of 13th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2020

Conference

Conference7th International Conference on Bioimaging, BIOIMAGING 2020 - Part of 13th International Joint Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2020
CountryMalta
CityValletta
Period24.02.202026.02.2020

Keywords

  • 3D microstructures
  • Bioinformatics
  • Fluorescence microscopy
  • Image analysis
  • Image processing methods
  • Software development

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