Proteins on Supported Lipid Bilayers Diffusing around Proteins Fixed on Acrylate Anchors

Bianca Buchegger, Johannes Kreutzer, Markus Axmann, Sandra Mayr, Richard Wollhofen, Birgit Plochberger, Jaroslaw Jacak, Thomas Klar

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Mobility of proteins and lipids plays a major role in physiological processes. Platforms which were developed to study protein interaction between immobilized and mobile proteins suffer from shortcomings such as fluorescence quenching or complicated fabrication methods. Here we report a versatile platform comprising immobilized histidine-tagged proteins and biotinylated proteins in a mobile phase. Importantly, multiphoton photolithography was used for easy and fast fabrication of the platform and allows, in principle, extension of its application to three dimensions. The platform, which is made up of functionalized polymer structures embedded in a mobile lipid bilayer, shows low background fluorescence and allows for mobility of arbitrary proteins.

Original languageEnglish
Pages (from-to)12372-12376
Number of pages5
JournalAnalytical Chemistry
Volume90
Issue number21
DOIs
Publication statusPublished - 6 Nov 2018

Keywords

  • Acrylates/chemistry
  • Diffusion
  • Fluorescence
  • Lipid Bilayers/chemistry
  • Photochemical Processes
  • Polymers/chemistry
  • Proteins/chemistry

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