@inbook{b1eb0079fd974175880e98d92c1bf5ad,
title = "Protein micropatterning assay: Quantitative analysis of protein–protein interactions",
abstract = "Characterization, especially quantification, of protein interactions in live cells is usually not an easy endeavor. Here, we describe a straightforward method to identify and quantify the interaction of a membrane protein (“bait”) and a fluorescently labeled interaction partner (“prey”) (membrane-bound or cyto-solic) in live cells using Total Internal Reflection Fluorescence microscopy. The bait protein is immobilized within patterns in the plasma membrane (e.g., via an antibody); the bait–prey interaction strength can be quantified by determining the prey bulk fluorescence intensity with respect to the bait patterns. This method is particularly suitable also for the analysis of weak, transient interactions that are not easily accessible with other methods.",
keywords = "Membrane proteins, Micropatterning, Protein–protein interactions, Quantitive analysis, Soft lithography, TIRF microscopy, Proteins/metabolism, Protein Interaction Mapping/methods, Workflow, Membrane Proteins/metabolism, Fluorescent Antibody Technique, Protein Binding, Microscopy, Fluorescence",
author = "Gerhard Sch{\"u}tz and Julian Weghuber and Peter Lanzerstorfer and Eva Sevcsik",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6747-6_18",
language = "English",
volume = "1550",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "261--270",
booktitle = "Methods in Molecular Biology",
}