Photoacoustic microscopy of single cells employing an intensity-modulated diode laser

Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Manoj Kumar Dasa, Thomas A. Klar, Thomas Berer

Research output: Chapter in Book/Report/Conference proceedingsConference contributionpeer-review


In this work, we employ frequency-domain photoacoustic microscopy to obtain photoacoustic images of labeled and unlabeled cells. The photoacoustic microscope is based on an intensity-modulated diode laser in combination with a focused piezo-composite transducer and allows imaging of labeled cells without severe photo-bleaching. We demonstrate that frequency-domain photoacoustic microscopy realized with a diode laser is capable of recording photoacoustic images of single cells with sub-μm resolution. As examples, we present images of undyed human red blood cells, stained human epithelial cells, and stained yeast cells.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2018
EditorsLihong V. Wang, Alexander A. Oraevsky
ISBN (Electronic)9781510614734
Publication statusPublished - 2018
EventPhotons Plus Ultrasound: Imaging and Sensing 2018 - San Francisco, United States
Duration: 28 Jan 20181 Feb 2018

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferencePhotons Plus Ultrasound: Imaging and Sensing 2018
Country/TerritoryUnited States
CitySan Francisco


  • diode laser
  • frequency-domain
  • photoacoustic microscopy


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