Tomography is an imaging method that records multiple slices and reconstructs the overall volume with the given information. Here we present a method for recording multiple 3D direct stochastic optical reconstruction microscopy (dSTORM) images with an axial height of 1 μm. Sequences are stacked together to receive reconstructed images of several micrometers height. The applied algorithms allow a lateral resolution of sub 35 nm and an axial resolution of approximate 90 nm by using optimized buffers.
|Title of host publication||Proceedings of the First INTERREG - 6th TERMIS Winterschool 2018|
|Publication status||Published - 2018|
|Event||First INTERREG - 6th TERMIS Winterschool 2018 - Radstadt, Salzburg, Austria|
Duration: 14 Jan 2018 → 17 Jan 2018
|Conference||First INTERREG - 6th TERMIS Winterschool 2018|
|Period||14.01.2018 → 17.01.2018|
- 3D dSTORM