Nanometer Tomography of Cells

Fabian Hauser, Sandra Mayr, Sujitha Puthukodan, Andreas Tauscher, Thomas Klar, Jaroslaw Jacak

Research output: Chapter in Book/Report/Conference proceedingsConference contribution

Abstract

Tomography is an imaging method that records multiple slices and reconstructs the overall volume with the given information. Here we present a method for recording multiple 3D direct stochastic optical reconstruction microscopy (dSTORM) images with an axial height of 1 μm. Sequences are stacked together to receive reconstructed images of several micrometers height. The applied algorithms allow a lateral resolution of sub 35 nm and an axial resolution of approximate 90 nm by using optimized buffers.
Original languageEnglish
Title of host publicationProceedings of the First INTERREG - 6th TERMIS Winterschool 2018
Publication statusPublished - 2018
EventFirst INTERREG - 6th TERMIS Winterschool 2018 - Radstadt, Salzburg, Austria
Duration: 14 Jan 201817 Jan 2018
http://trauma.lbg.ac.at/en/invitation-1st-interreg-6th-termis-winterschool-2018-extended-deadline

Conference

ConferenceFirst INTERREG - 6th TERMIS Winterschool 2018
CountryAustria
CityRadstadt, Salzburg
Period14.01.201817.01.2018
Internet address

Keywords

  • 3D dSTORM
  • Cells
  • Actin
  • Tomography

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