Nanometer Tomography of Cells

Fabian Hauser; Sandra Mayr; Sujitha Puthukodan; Andreas Tauscher; Thomas Klar; Jaroslaw Jacak

Nanometer Tomography of Cells

Fabian Hauser, Sandra Mayr, Sujitha Puthukodan, Andreas Tauscher, Thomas Klar, Jaroslaw Jacak

Research output: Chapter in Book/Report/Conference proceedings › Conference contribution

Abstract

Tomography is an imaging method that records multiple slices and reconstructs the overall volume with the given information. Here we present a method for recording multiple 3D direct stochastic optical reconstruction microscopy (dSTORM) images with an axial height of 1 μm. Sequences are stacked together to receive reconstructed images of several micrometers height. The applied algorithms allow a lateral resolution of sub 35 nm and an axial resolution of approximate 90 nm by using optimized buffers.

Original language	English
Title of host publication	Proceedings of the First INTERREG - 6th TERMIS Winterschool 2018
Publication status	Published - 2018
Event	First INTERREG - 6th TERMIS Winterschool 2018 - Radstadt, Salzburg, Austria Duration: 14 Jan 2018 → 17 Jan 2018 http://trauma.lbg.ac.at/en/invitation-1st-interreg-6th-termis-winterschool-2018-extended-deadline

Conference

Conference	First INTERREG - 6th TERMIS Winterschool 2018
Country/Territory	Austria
City	Radstadt, Salzburg
Period	14.01.2018 → 17.01.2018
Internet address	http://trauma.lbg.ac.at/en/invitation-1st-interreg-6th-termis-winterschool-2018-extended-deadline

Keywords

3D dSTORM
Cells
Actin
Tomography

Cite this

@inproceedings{cef50e1aabb2437f958485dd1f11f9a0,

title = "Nanometer Tomography of Cells",

abstract = "Tomography is an imaging method that records multiple slices and reconstructs the overall volume with the given information. Here we present a method for recording multiple 3D direct stochastic optical reconstruction microscopy (dSTORM) images with an axial height of 1 μm. Sequences are stacked together to receive reconstructed images of several micrometers height. The applied algorithms allow a lateral resolution of sub 35 nm and an axial resolution of approximate 90 nm by using optimized buffers.",

keywords = "3D dSTORM, Cells, Actin, Tomography, 3D dSTORM, Cells, Actin, Tomography",

author = "Fabian Hauser and Sandra Mayr and Sujitha Puthukodan and Andreas Tauscher and Thomas Klar and Jaroslaw Jacak",

year = "2018",

language = "English",

booktitle = "Proceedings of the First INTERREG - 6th TERMIS Winterschool 2018",

note = "First INTERREG - 6th TERMIS Winterschool 2018 ; Conference date: 14-01-2018 Through 17-01-2018",

url = "http://trauma.lbg.ac.at/en/invitation-1st-interreg-6th-termis-winterschool-2018-extended-deadline",

}

TY - GEN

T1 - Nanometer Tomography of Cells

AU - Hauser, Fabian

AU - Mayr, Sandra

AU - Puthukodan, Sujitha

AU - Tauscher, Andreas

AU - Klar, Thomas

AU - Jacak, Jaroslaw

PY - 2018

Y1 - 2018

N2 - Tomography is an imaging method that records multiple slices and reconstructs the overall volume with the given information. Here we present a method for recording multiple 3D direct stochastic optical reconstruction microscopy (dSTORM) images with an axial height of 1 μm. Sequences are stacked together to receive reconstructed images of several micrometers height. The applied algorithms allow a lateral resolution of sub 35 nm and an axial resolution of approximate 90 nm by using optimized buffers.

AB - Tomography is an imaging method that records multiple slices and reconstructs the overall volume with the given information. Here we present a method for recording multiple 3D direct stochastic optical reconstruction microscopy (dSTORM) images with an axial height of 1 μm. Sequences are stacked together to receive reconstructed images of several micrometers height. The applied algorithms allow a lateral resolution of sub 35 nm and an axial resolution of approximate 90 nm by using optimized buffers.

KW - 3D dSTORM

KW - Cells

KW - Actin

KW - Tomography

KW - 3D dSTORM

KW - Cells

KW - Actin

KW - Tomography

M3 - Conference contribution

BT - Proceedings of the First INTERREG - 6th TERMIS Winterschool 2018

T2 - First INTERREG - 6th TERMIS Winterschool 2018

Y2 - 14 January 2018 through 17 January 2018

ER -

Nanometer Tomography of Cells

Abstract

Conference

Keywords

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Cite this