Nano-anchors with single protein capacity produced with STED lithography

Jaroslaw Jacak, Moritz Wiesbauer, Richard Wollhofen, Borislav Vasic, Kurt Schilcher, Thomas Klar

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Acrylate nanoanchors of subdiffraction-limited diameter are written with optical stimulated emission depletion (STED) lithography. After incubation, 98% of all nanoanchors are loaded quickly with fluorescently labeled antibodies. Controlling the size of the nanoanchors allows for limiting the number of the antibodies. Direct stochastic optical reconstruction microscopy (dSTORM) imaging, statistical distribution of fluorescence, quantitative fluorescence readout, and single molecule blinking consistently prove that 80% of the nanoanchors with a 65 nm diameter are carrying only one antibody each, which are functional as confirmed with live erythrocytes.

Original languageEnglish
Pages (from-to)5672-5678
Number of pages7
JournalNano Letters
Volume13
Issue number11
DOIs
Publication statusPublished - 13 Nov 2013

Keywords

  • polymer nanostructures
  • single molecule imaging
  • single protein array
  • STED lithography
  • STED microscopy
  • STORM imaging
  • Humans
  • Nanostructures
  • Fluorescent Dyes/chemistry
  • Image Processing, Computer-Assisted
  • Acrylates/chemistry
  • Antibodies/chemistry
  • Erythrocytes/immunology
  • Microscopy, Fluorescence
  • Nanotechnology

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