Nano-anchors with single protein capacity produced with STED lithography

Jaroslaw Jacak; Moritz Wiesbauer; Richard Wollhofen; Borislav Vasic; Kurt Schilcher; Thomas Klar

doi:10.1021/nl4033523

Nano-anchors with single protein capacity produced with STED lithography

Jaroslaw Jacak, Moritz Wiesbauer, Richard Wollhofen, Borislav Vasic, Kurt Schilcher, Thomas Klar

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

Acrylate nanoanchors of subdiffraction-limited diameter are written with optical stimulated emission depletion (STED) lithography. After incubation, 98% of all nanoanchors are loaded quickly with fluorescently labeled antibodies. Controlling the size of the nanoanchors allows for limiting the number of the antibodies. Direct stochastic optical reconstruction microscopy (dSTORM) imaging, statistical distribution of fluorescence, quantitative fluorescence readout, and single molecule blinking consistently prove that 80% of the nanoanchors with a 65 nm diameter are carrying only one antibody each, which are functional as confirmed with live erythrocytes.

Original language	English
Pages (from-to)	5672-5678
Number of pages	7
Journal	Nano Letters
Volume	13
Issue number	11
DOIs	https://doi.org/10.1021/nl4033523
Publication status	Published - 13 Nov 2013

Keywords

polymer nanostructures
single molecule imaging
single protein array
STED lithography
STED microscopy
STORM imaging
Humans
Nanostructures
Fluorescent Dyes/chemistry
Image Processing, Computer-Assisted
Acrylates/chemistry
Antibodies/chemistry
Erythrocytes/immunology
Microscopy, Fluorescence
Nanotechnology

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10.1021/nl4033523

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title = "Nano-anchors with single protein capacity produced with STED lithography",

abstract = "Acrylate nanoanchors of subdiffraction-limited diameter are written with optical stimulated emission depletion (STED) lithography. After incubation, 98% of all nanoanchors are loaded quickly with fluorescently labeled antibodies. Controlling the size of the nanoanchors allows for limiting the number of the antibodies. Direct stochastic optical reconstruction microscopy (dSTORM) imaging, statistical distribution of fluorescence, quantitative fluorescence readout, and single molecule blinking consistently prove that 80% of the nanoanchors with a 65 nm diameter are carrying only one antibody each, which are functional as confirmed with live erythrocytes.",

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author = "Jaroslaw Jacak and Moritz Wiesbauer and Richard Wollhofen and Borislav Vasic and Kurt Schilcher and Thomas Klar",

year = "2013",

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doi = "10.1021/nl4033523",

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journal = "Nano Letters",

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T1 - Nano-anchors with single protein capacity produced with STED lithography

AU - Jacak, Jaroslaw

AU - Wiesbauer, Moritz

AU - Wollhofen, Richard

AU - Vasic, Borislav

AU - Schilcher, Kurt

AU - Klar, Thomas

PY - 2013/11/13

Y1 - 2013/11/13

N2 - Acrylate nanoanchors of subdiffraction-limited diameter are written with optical stimulated emission depletion (STED) lithography. After incubation, 98% of all nanoanchors are loaded quickly with fluorescently labeled antibodies. Controlling the size of the nanoanchors allows for limiting the number of the antibodies. Direct stochastic optical reconstruction microscopy (dSTORM) imaging, statistical distribution of fluorescence, quantitative fluorescence readout, and single molecule blinking consistently prove that 80% of the nanoanchors with a 65 nm diameter are carrying only one antibody each, which are functional as confirmed with live erythrocytes.

AB - Acrylate nanoanchors of subdiffraction-limited diameter are written with optical stimulated emission depletion (STED) lithography. After incubation, 98% of all nanoanchors are loaded quickly with fluorescently labeled antibodies. Controlling the size of the nanoanchors allows for limiting the number of the antibodies. Direct stochastic optical reconstruction microscopy (dSTORM) imaging, statistical distribution of fluorescence, quantitative fluorescence readout, and single molecule blinking consistently prove that 80% of the nanoanchors with a 65 nm diameter are carrying only one antibody each, which are functional as confirmed with live erythrocytes.

KW - polymer nanostructures

KW - single molecule imaging

KW - single protein array

KW - STED lithography

KW - STED microscopy

KW - STORM imaging

KW - Humans

KW - Nanostructures

KW - Fluorescent Dyes/chemistry

KW - Image Processing, Computer-Assisted

KW - Acrylates/chemistry

KW - Antibodies/chemistry

KW - Erythrocytes/immunology

KW - Microscopy, Fluorescence

KW - Nanotechnology

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DO - 10.1021/nl4033523

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SP - 5672

EP - 5678

JO - Nano Letters

JF - Nano Letters

IS - 11

ER -

Nano-anchors with single protein capacity produced with STED lithography

Abstract

Keywords

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