Multimodal fluorescence and photoacoustic microscopy in the frequency domain

Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Thomas Klar, Thomas Berer

Research output: Chapter in Book/Report/Conference proceedingsConference contributionpeer-review

4 Citations (Scopus)


In this paper a multimodal optical resolution microscope in frequency domain is introduced. Fluorescence and photoacoustic signals are generated simultaneously using an amplitude modulated diode laser. The resulting signals are recorded by a lock-in amplifier. By scanning the sample, two-dimensional plots of the fluorescence and photoacoustic signals can be generated. In this paper, the signal-to-noise ratios of amplitude modulated signals are compared to those expected for pulsed excitation under the assumption that the maximum permissible exposure limits are fulfilled. Furthermore, it is described how to determine the excited state life-times from the measured frequency responses of the fluorescence or photoacoustic signals, respectively.

Original languageEnglish
Title of host publicationMultimodal Biomedical Imaging XII
EditorsFred S. Azar, Xavier Intes
PublisherAmerican Society for Cell Biology
ISBN (Electronic)9781510605558
Publication statusPublished - 2017
EventSPIE BiOS 2017 - San Francisco, United States
Duration: 28 Jan 20172 Feb 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceSPIE BiOS 2017
Country/TerritoryUnited States
CitySan Francisco


  • Fluorescence microscopy
  • Frequency domain
  • Multimodal microscopy
  • Photoacoustic microscopy


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