Measuring colocalization by dual color single molecule imaging. Thresholds, error rates, and sensitivity

Verena Ruprecht; Julian Weghuber; Stefan Wieser; Gerhard J. Schütz

doi:10.1016/B978-0-12-381266-7.00002-X

Measuring colocalization by dual color single molecule imaging. Thresholds, error rates, and sensitivity

Verena Ruprecht, Julian Weghuber, Stefan Wieser, Gerhard J. Schütz

Research Center Wels

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Dual color single molecule tracking is a state-of-the-art fluorescence microscopy technique to study molecular associations. Featuring high spatial and temporal resolution, the method allows for catching associations with extremely high reliability. In this review, we describe robust data analysis approaches to identify and characterize molecular associations from dual color fluorescence images and show how colocalization can be statistically discriminated from accidental colocalizations. We discriminate the case of static analysis where colocalizations are revealed from individual dual color fluorescence images, and the case of dynamic analysis where colocalizations are tracked in time.

Original language	English
Pages (from-to)	21-40
Number of pages	20
Journal	Advances in Biomembranes and Lipid Self-Assembly
Volume	12
Issue number	C
DOIs	https://doi.org/10.1016/B978-0-12-381266-7.00002-X
Publication status	Published - 2010

Keywords

Association
Diffusion
Fluorescence
Plasma membrane
Protein interaction
Single molecule microscopy
Single particle tracking

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10.1016/B978-0-12-381266-7.00002-X

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abstract = "Dual color single molecule tracking is a state-of-the-art fluorescence microscopy technique to study molecular associations. Featuring high spatial and temporal resolution, the method allows for catching associations with extremely high reliability. In this review, we describe robust data analysis approaches to identify and characterize molecular associations from dual color fluorescence images and show how colocalization can be statistically discriminated from accidental colocalizations. We discriminate the case of static analysis where colocalizations are revealed from individual dual color fluorescence images, and the case of dynamic analysis where colocalizations are tracked in time.",

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AU - Ruprecht, Verena

AU - Weghuber, Julian

AU - Wieser, Stefan

AU - Schütz, Gerhard J.

PY - 2010

Y1 - 2010

N2 - Dual color single molecule tracking is a state-of-the-art fluorescence microscopy technique to study molecular associations. Featuring high spatial and temporal resolution, the method allows for catching associations with extremely high reliability. In this review, we describe robust data analysis approaches to identify and characterize molecular associations from dual color fluorescence images and show how colocalization can be statistically discriminated from accidental colocalizations. We discriminate the case of static analysis where colocalizations are revealed from individual dual color fluorescence images, and the case of dynamic analysis where colocalizations are tracked in time.

AB - Dual color single molecule tracking is a state-of-the-art fluorescence microscopy technique to study molecular associations. Featuring high spatial and temporal resolution, the method allows for catching associations with extremely high reliability. In this review, we describe robust data analysis approaches to identify and characterize molecular associations from dual color fluorescence images and show how colocalization can be statistically discriminated from accidental colocalizations. We discriminate the case of static analysis where colocalizations are revealed from individual dual color fluorescence images, and the case of dynamic analysis where colocalizations are tracked in time.

KW - Association

KW - Diffusion

KW - Fluorescence

KW - Plasma membrane

KW - Protein interaction

KW - Single molecule microscopy

KW - Single particle tracking

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SN - 2451-9634

VL - 12

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EP - 40

JO - Advances in Biomembranes and Lipid Self-Assembly

JF - Advances in Biomembranes and Lipid Self-Assembly

IS - C

ER -

Measuring colocalization by dual color single molecule imaging. Thresholds, error rates, and sensitivity

Abstract

Keywords

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