Abstract
The segregation in dried droplet MALDI sample spots was analyzed with regard to the matrix-to-sample ratio using optical microscopy, MALDI imaging mass spectrometry (MALDI MSI) and IR imaging spectroscopy. In this context, different polymer/matrix/solvent systems usually applied in the analysis of synthetic polymers were investigated. The use of typical matrix concentrations (10 mg mL-1) in almost every case resulted in ring patterns, whereas higher concentrated matrix solutions always led to homogeneous sample spot layers. The data revealed that segregation is predominantly caused by matrix transport in the drying droplet, whereas polymer segregation seems to be only secondary.
| Original language | English |
|---|---|
| Pages (from-to) | 1356-1363 |
| Number of pages | 8 |
| Journal | Journal of the American Society for Mass Spectrometry |
| Volume | 25 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 2014 |
| Externally published | Yes |
Keywords
- Dried droplet
- Imaging MS
- MALDI
- Matrix segregation
- Polymers
- Sample preparation
- Optical Imaging
- Nitriles/chemistry
- Molecular Weight
- Gentisates/chemistry
- Polymethyl Methacrylate/chemistry
- Tretinoin/chemistry
- Spectrophotometry, Infrared
- Volatilization
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Furans/chemistry
- Polystyrenes/chemistry
- Reproducibility of Results
- Electrochemical Techniques
- Membranes, Artificial
- Models, Molecular
- Acetonitriles/chemistry
- Solvents/chemistry
- Microscopy
- Polyethylene Glycols/chemistry