Matrix segregation as the major cause for sample inhomogeneity in MALDI dried droplet spots

Stefan J. Gabriel, Clemens Schwarzinger, Bettina Schwarzinger, Ulrich Panne, Steffen M. Weidner

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)


The segregation in dried droplet MALDI sample spots was analyzed with regard to the matrix-to-sample ratio using optical microscopy, MALDI imaging mass spectrometry (MALDI MSI) and IR imaging spectroscopy. In this context, different polymer/matrix/solvent systems usually applied in the analysis of synthetic polymers were investigated. The use of typical matrix concentrations (10 mg mL-1) in almost every case resulted in ring patterns, whereas higher concentrated matrix solutions always led to homogeneous sample spot layers. The data revealed that segregation is predominantly caused by matrix transport in the drying droplet, whereas polymer segregation seems to be only secondary.

Original languageEnglish
Pages (from-to)1356-1363
Number of pages8
JournalJournal of the American Society for Mass Spectrometry
Issue number8
Publication statusPublished - Aug 2014
Externally publishedYes


  • Dried droplet
  • Imaging MS
  • Matrix segregation
  • Polymers
  • Sample preparation
  • Optical Imaging
  • Nitriles/chemistry
  • Molecular Weight
  • Gentisates/chemistry
  • Polymethyl Methacrylate/chemistry
  • Tretinoin/chemistry
  • Spectrophotometry, Infrared
  • Volatilization
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Furans/chemistry
  • Polystyrenes/chemistry
  • Reproducibility of Results
  • Electrochemical Techniques
  • Membranes, Artificial
  • Models, Molecular
  • Acetonitriles/chemistry
  • Solvents/chemistry
  • Microscopy
  • Polyethylene Glycols/chemistry


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