Localization Microscopy of Actin Cytoskeleton in Human Platelets

Sandra Mayr, Fabian Hauser, Anja Peterbauer, Andreas Tauscher, Christoph Naderer, Markus Axmann, Birgit Plochberger, Jaroslaw Jacak

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional accuracy of 12 nm
Original languageEnglish
Article number1150
JournalInternational Journal of Molecular Sciences
Issue number4
Publication statusPublished - 11 Apr 2018


  • actin cytoskeleton
  • Alexa Fluor 488
  • drift correction
  • dSTORM
  • localization microscopy
  • rhodamine
  • photo-switching
  • platelet shape change
  • Platelet shape change
  • Rhodamine
  • Drift correction
  • Photo-switching
  • Localization microscopy
  • Actin cytoskeleton
  • Actins/metabolism
  • Humans
  • Cells, Cultured
  • Fluorescent Dyes/chemistry
  • Blood Platelets/metabolism
  • Sensitivity and Specificity
  • Microscopy, Fluorescence/methods


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