Abstract
Here, we measure the actin cytoskeleton arrangement of different morphological states
of human platelets using a new protocol for photo-switching of rhodamine class fluorophores.
A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488
conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized
and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less
drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional
accuracy of 12 nm
| Original language | English |
|---|---|
| Article number | 1150 |
| Journal | International Journal of Molecular Sciences |
| Volume | 19 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 11 Apr 2018 |
Keywords
- actin cytoskeleton
- Alexa Fluor 488
- drift correction
- dSTORM
- localization microscopy
- rhodamine
- photo-switching
- platelet shape change
- Platelet shape change
- Rhodamine
- Drift correction
- DSTORM
- Photo-switching
- Localization microscopy
- Actin cytoskeleton
- Actins/metabolism
- Humans
- Cells, Cultured
- Fluorescent Dyes/chemistry
- Blood Platelets/metabolism
- Sensitivity and Specificity
- Microscopy, Fluorescence/methods