Lipoprotein Particles as Shuttles for Hydrophilic Cargo

Florian Weber; Markus Axmann; Andreas Horner; Bettina Schwarzinger; Julian Weghuber; Birgit Plochberger

doi:10.3390/membranes13050471

Lipoprotein Particles as Shuttles for Hydrophilic Cargo

Florian Weber, Markus Axmann, Andreas Horner, Bettina Schwarzinger, Julian Weghuber, Birgit Plochberger

Research output: Contribution to journal › Article › peer-review

Abstract

Lipoprotein particles (LPs) are excellent transporters and have been intensively studied in cardiovascular diseases, especially regarding parameters such as their class distribution and accumulation, site-specific delivery, cellular internalization, and escape from endo/lysosomal compartments. The aim of the present work is the hydrophilic cargo loading of LPs. As an exemplary proof-of-principle showcase, the glucose metabolism-regulating hormone, insulin, was successfully incorporated into high-density lipoprotein (HDL) particles. The incorporation was studied and verified to be successful using Atomic Force Microscopy (AFM) and Fluorescence Microscopy (FM). Single-molecule-sensitive FM together with confocal imaging visualized the membrane interaction of single, insulin-loaded HDL particles and the subsequent cellular translocation of glucose transporter type 4 (Glut4).

Original language	English
Article number	471
Journal	Membranes
Volume	13
Issue number	5
DOIs	https://doi.org/10.3390/membranes13050471
Publication status	Published - 28 Apr 2023

Keywords

atomic force microscopy
insulin
lipoprotein particles
shuttle for hydrophilic cargo
single-molecule-sensitive fluorescence microscopy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/membranes13050471

Cite this

@article{1b570145692e46f890f86a89fbda979f,

title = "Lipoprotein Particles as Shuttles for Hydrophilic Cargo",

abstract = "Lipoprotein particles (LPs) are excellent transporters and have been intensively studied in cardiovascular diseases, especially regarding parameters such as their class distribution and accumulation, site-specific delivery, cellular internalization, and escape from endo/lysosomal compartments. The aim of the present work is the hydrophilic cargo loading of LPs. As an exemplary proof-of-principle showcase, the glucose metabolism-regulating hormone, insulin, was successfully incorporated into high-density lipoprotein (HDL) particles. The incorporation was studied and verified to be successful using Atomic Force Microscopy (AFM) and Fluorescence Microscopy (FM). Single-molecule-sensitive FM together with confocal imaging visualized the membrane interaction of single, insulin-loaded HDL particles and the subsequent cellular translocation of glucose transporter type 4 (Glut4).",

keywords = "atomic force microscopy, insulin, lipoprotein particles, shuttle for hydrophilic cargo, single-molecule-sensitive fluorescence microscopy",

author = "Florian Weber and Markus Axmann and Andreas Horner and Bettina Schwarzinger and Julian Weghuber and Birgit Plochberger",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = apr,

day = "28",

doi = "10.3390/membranes13050471",

language = "English",

volume = "13",

journal = "Membranes",

issn = "2077-0375",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "5",

}

TY - JOUR

T1 - Lipoprotein Particles as Shuttles for Hydrophilic Cargo

AU - Weber, Florian

AU - Axmann, Markus

AU - Horner, Andreas

AU - Schwarzinger, Bettina

AU - Weghuber, Julian

AU - Plochberger, Birgit

PY - 2023/4/28

Y1 - 2023/4/28

N2 - Lipoprotein particles (LPs) are excellent transporters and have been intensively studied in cardiovascular diseases, especially regarding parameters such as their class distribution and accumulation, site-specific delivery, cellular internalization, and escape from endo/lysosomal compartments. The aim of the present work is the hydrophilic cargo loading of LPs. As an exemplary proof-of-principle showcase, the glucose metabolism-regulating hormone, insulin, was successfully incorporated into high-density lipoprotein (HDL) particles. The incorporation was studied and verified to be successful using Atomic Force Microscopy (AFM) and Fluorescence Microscopy (FM). Single-molecule-sensitive FM together with confocal imaging visualized the membrane interaction of single, insulin-loaded HDL particles and the subsequent cellular translocation of glucose transporter type 4 (Glut4).

AB - Lipoprotein particles (LPs) are excellent transporters and have been intensively studied in cardiovascular diseases, especially regarding parameters such as their class distribution and accumulation, site-specific delivery, cellular internalization, and escape from endo/lysosomal compartments. The aim of the present work is the hydrophilic cargo loading of LPs. As an exemplary proof-of-principle showcase, the glucose metabolism-regulating hormone, insulin, was successfully incorporated into high-density lipoprotein (HDL) particles. The incorporation was studied and verified to be successful using Atomic Force Microscopy (AFM) and Fluorescence Microscopy (FM). Single-molecule-sensitive FM together with confocal imaging visualized the membrane interaction of single, insulin-loaded HDL particles and the subsequent cellular translocation of glucose transporter type 4 (Glut4).

KW - atomic force microscopy

KW - insulin

KW - lipoprotein particles

KW - shuttle for hydrophilic cargo

KW - single-molecule-sensitive fluorescence microscopy

UR - http://www.scopus.com/inward/record.url?scp=85160362044&partnerID=8YFLogxK

U2 - 10.3390/membranes13050471

DO - 10.3390/membranes13050471

M3 - Article

C2 - 37233532

AN - SCOPUS:85160362044

SN - 2077-0375

VL - 13

JO - Membranes

JF - Membranes

IS - 5

M1 - 471

ER -

Lipoprotein Particles as Shuttles for Hydrophilic Cargo

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this