IN-VITRO ANTIDIABETIC ACTIVITY OF A BISTORTA OFFICINALIS DELARBRE ROOT EXTRACT CAN NOT BE CONFIRMED IN THE IN-VIVO MODELS HEN’S EGG TEST AND DROSOPHILA MELANOGASTER

Ilka Bauer, G. Rimbach, S. Nevermann, C. Neuhauser, B. Schwarzinger, C. Schwarzinger, J. Weghuber, K. Luersen

Research output: Contribution to journalArticlepeer-review

Abstract

The potential of plant bioactives for the prevention and therapy of diabetes is increasingly being recognized. In the present study we investigated the antidiabetic properties of an aqueous Bistorta officinalis Delarbre extract (BODE) by employing both in-vitro assays and in-vivo models. Multiple targets in glucose homeostasis which are involved in the regulation of the blood glucose level were affected by BODE in-vitro. The extract exhibited inhibitory activities towards the intestinal carbohydrate-hydrolysing enzymes α-amylase and α-glucosidase with IC50 values of 81.5 µg/mL and 8.4 µg/mL, respectively. Furthermore, moderate reduction of the dipeptidyl peptidase-4 (DPP4) enzyme activity was evident when tested in the presence of 1.0 mg/mL BODE. A significant inhibition of the intestinal glucose transporter sodium-dependent glucose transporter 1 (SGLT1) in response to 1.0 mg/mL BODE was shown for Caco-2 cells mounted in Ussing chambers. High performance liquid chromatography-mass spectrometry analyses of the BODE revealed several plant bioactives including gallotannins, catechins and chlorogenic acid. Although our in-vitro data were promising, BODE-supplementation in the model organism Drosophila melanogaster lacked to confirm the antidiabetic effect of the extract in-vivo. Moreover, BODE failed to reduce blood glucose levels in chicken embryos (in-ovo). Hence, BODE is probably not a suitable candidate for developing a pharmaceutical against diabetes mellitus.

Original languageEnglish
Pages (from-to)31-42
Number of pages12
JournalJournal of Physiology and Pharmacology
Volume74
Issue number1
DOIs
Publication statusPublished - Feb 2023

Keywords

  • Bistorta officinalis Delarbre
  • diabetes mellitus
  • dipeptidyl peptidase-4
  • Drosophila melanogaster
  • hen’s egg test
  • sodium-dependent glucose transporter 1
  • α-amylase inhibition
  • α-glucosidase inhibition
  • Caco-2 Cells
  • Humans
  • Blood Glucose
  • Diabetes Mellitus
  • Chick Embryo
  • Animals
  • Chickens
  • Female
  • Plant Extracts/pharmacology
  • Hypoglycemic Agents/pharmacology

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