In vitro and in vivo inhibition of intestinal glucose transport by guava (Psidium guajava) extracts

Ulrike Müller, Flora Stübl, Bettina Schwarzinger, Georg Philipp Sandner, Marcus Iken, Markus Himmelsbach, Clemens Schwarzinger, Nicole Ollinger, Verena Stadlbauer, Otmar Höglinger, Peter Lanzerstorfer, Julian Weghuber

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Scope: Known pharmacological activities of guava (Psidium guajava) include modulation of blood glucose levels. However, mechanistic details remain unclear in many cases. Methods and results: This study investigated the effects of different guava leaf and fruit extracts on intestinal glucose transport in vitro and on postprandial glucose levels in vivo. Substantial dose- and time-dependent glucose transport inhibition (up to 80%) was observed for both guava fruit and leaf extracts, at conceivable physiological concentrations in Caco-2 cells. Using sodium-containing (both glucose transporters, sodium-dependent glucose transporter 1 [SGLT1] and glucose transporter 2 [GLUT2], are active) and sodium-free (only GLUT2 is active) conditions, we show that inhibition of GLUT2 was greater than that of SGLT1. Inhibitory properties of guava extracts also remained stable after digestive juice treatment, indicating a good chemical stability of the active substances. Furthermore, we could unequivocally show that guava extracts significantly reduced blood glucose levels (≈fourfold reduction) in a time-dependent manner in vivo (C57BL/6N mice). Extracts were characterized with respect to their main putative bioactive compounds (polyphenols) using HPLC and LC-MS. Conclusion: The data demonstrated that guava leaf and fruit extracts can potentially contribute to the regulation of blood glucose levels.

Original languageEnglish
Article number1701012
JournalMolecular nutrition and food research
Volume62
Issue number11
DOIs
Publication statusPublished - Jun 2018

Keywords

  • GLUT2
  • SGLT1
  • guava extract
  • intestinal glucose transport
  • postprandial blood glucose
  • Caco-2 Cells
  • Sodium-Glucose Transporter 1/genetics
  • Humans
  • Mice, Inbred C57BL
  • Biological Transport/drug effects
  • Polyphenols/analysis
  • Fruit/chemistry
  • Glucose Transporter Type 5/genetics
  • Intestinal Mucosa/drug effects
  • Plant Extracts/analysis
  • Animals
  • Psidium/chemistry
  • Plant Leaves/chemistry
  • Postprandial Period
  • Glucose Transporter Type 2/genetics
  • Female
  • Glucose/metabolism
  • Hypoglycemic Agents/pharmacology

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