IL-1β Induces SOCS2 Expression in Human Dendritic Cells

Muamera Sarajlic, Theresa Neuper, Kim Tamara Föhrenbach Quiroz, Sara Michelini, Julia Vetter, Susanne Schaller, Jutta Horejs-Hoeck

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Dendritic cells (DCs) regulate immunity and inflammation and respond to various stimuli, including cytokines. IL-1β is a key cytokine in the course of both acute and chronic inflammatory responses, making it indispensable for protection of the host, but also linking it to several diseases. Thus, IL-1β signaling must be tightly regulated. As suppressor of cytokine signaling (SOCS) proteins effectively control immune responses, we investigated the role of SOCS2 in IL-1β-induced DC activation. Human monocyte-derived DCs were stimulated with IL-1β, and SOCS2 mRNA and protein levels were measured. DC activation was assessed by cytokine secretion and surface marker expression. For functional analysis, small interfering RNA (siRNA)-based SOCS2 silencing was performed. SOCS2 expression was also analyzed in a curated NCBI GEO dataset of myeloid leukemia patients. We found IL-1β to be a potent inducer of SOCS2 expression. By silencing SOCS2, we showed that SOCS2 specifically limits IL-1β-induced IL-8 secretion. Moreover, our analysis revealed that SOCS2 levels are significantly increased in patients with acute and chronic myeloid leukemia, two hematological malignancies where disease progression is closely linked to IL-1β. This study identifies SOCS2 as a novel IL-1β-inducible target gene and points toward a potential role of SOCS2 in IL-1β-mediated DC activation.

Original languageEnglish
Article number5931
JournalInternational Journal of Molecular Sciences
Issue number23
Publication statusPublished - 1 Dec 2019


  • Dendritic cell
  • IL-1β
  • SOCS2
  • Lymphocyte Activation/drug effects
  • Signal Transduction
  • Dendritic Cells/cytology
  • Humans
  • Cells, Cultured
  • Interleukin-1beta/pharmacology
  • Suppressor of Cytokine Signaling Proteins/genetics
  • Monocytes/cytology
  • Cell Differentiation
  • Cytokines/metabolism


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