Hsa-miR-3651 could serve as a novel predictor for in-breast recurrence via FRMD3

Barbara Zellinger, Ulrich Bodenhofer, Immanuela A. Engländer, Cornelia Kronberger, Brane Grambozov, Elvis Ruznic, Markus Stana, Josef Karner, Gerd Fastner, Karl Sotlar, Felix Sedlmayer, Franz Zehentmayr

Research output: Contribution to journalArticlepeer-review

Abstract

Background: MicroRNAs are small non-coding RNAs with pivotal regulatory functions in multiple cellular processes. Their significance as molecular predictors for breast cancer was demonstrated in the past 15 years. The aim of this study was to elucidate the role of hsa-miR-3651 for predicting of local control (LC) in early breast cancer. Results: By means of high-throughput technology, hsa-miR-3651 was found to be differentially expressed between patients who experienced local relapse compared to those without (N = 23; p = 0.0035). This result could be validated in an independent cohort of 87 patients using RT-qPCR (p < 0.0005). In a second analysis step with a chip-based microarray containing 70,523 probes of potential target molecules, FERM domain protein 3 (FRMD3) was found to be the most down-regulated protein (N = 21; p = 0.0016). Computational analysis employing different prediction algorithms revealed FRMD3 as a likely downstream target of hsa-miR-3651 with an 8mer binding site between the two molecules. This could be validated in an independent patient set (N = 20, p = 0.134). Conclusion: The current study revealed that hsa-miR-3651 is a predictor of LC in early breast cancer via its putative target protein FRMD3. Since microRNAs interfere in multiple pathways, the results of this hypothesis generating study may contribute to the development of tailored therapies for breast cancer in the future.

Original languageEnglish
Pages (from-to)274-286
Number of pages13
JournalBreast Cancer
Volume29
Issue number2
DOIs
Publication statusPublished - Mar 2022

Keywords

  • Early breast cancer
  • FRMD3
  • Hsa-miR-3651
  • Local control
  • Prediction
  • MicroRNAs/genetics
  • Oligonucleotide Array Sequence Analysis
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Gene Expression Profiling
  • Neoplasm Recurrence, Local/genetics
  • Female
  • Breast Neoplasms/genetics
  • Tumor Suppressor Proteins/genetics

Fingerprint

Dive into the research topics of 'Hsa-miR-3651 could serve as a novel predictor for in-breast recurrence via FRMD3'. Together they form a unique fingerprint.

Cite this