High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler

Taras Sych; Jan Schlegel; Hanna M.G. Barriga; Miina Ojansivu; Leo Hanke; Florian Weber; R. Beklem Bostancioglu; Kariem Ezzat; Herbert Stangl; Birgit Plochberger; Jurga Laurencikiene; Samir El Andaloussi; Daniel Fürth; Molly M. Stevens; Erdinc Sezgin

doi:10.1038/s41587-023-01825-5

High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler

Taras Sych
, Jan Schlegel
, Hanna M.G. Barriga
, Miina Ojansivu
, Leo Hanke
, Florian Weber
, R. Beklem Bostancioglu
, Kariem Ezzat
, Herbert Stangl
, Birgit Plochberger
, Jurga Laurencikiene
, Samir El Andaloussi
, Daniel Fürth
, Molly M. Stevens
, Erdinc Sezgin^*

^*Corresponding author for this work

Research Center Linz

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5–200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.

Original language	English
Pages (from-to)	587-590
Number of pages	4
Journal	Nature Biotechnology
Volume	42
Issue number	4
Early online date	Jun 2023
DOIs	https://doi.org/10.1038/s41587-023-01825-5
Publication status	Published - Apr 2024

Keywords

Particle Size
Liposomes/chemistry
Nanoparticles/chemistry

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10.1038/s41587-023-01825-5

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Sych, T., Schlegel, J., Barriga, H. M. G., Ojansivu, M., Hanke, L., Weber, F., Beklem Bostancioglu, R., Ezzat, K., Stangl, H., Plochberger, B., Laurencikiene, J., El Andaloussi, S., Fürth, D., Stevens, M. M., & Sezgin, E. (2024). High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler. Nature Biotechnology, 42(4), 587-590. https://doi.org/10.1038/s41587-023-01825-5

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title = "High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler",

abstract = "We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5–200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.",

keywords = "Particle Size, Liposomes/chemistry, Nanoparticles/chemistry",

author = "Taras Sych and Jan Schlegel and Barriga, \{Hanna M.G.\} and Miina Ojansivu and Leo Hanke and Florian Weber and \{Beklem Bostancioglu\}, R. and Kariem Ezzat and Herbert Stangl and Birgit Plochberger and Jurga Laurencikiene and \{El Andaloussi\}, Samir and Daniel F{\"u}rth and Stevens, \{Molly M.\} and Erdinc Sezgin",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2023.",

year = "2024",

month = apr,

doi = "10.1038/s41587-023-01825-5",

language = "English",

volume = "42",

pages = "587--590",

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Sych, T, Schlegel, J, Barriga, HMG, Ojansivu, M, Hanke, L, Weber, F, Beklem Bostancioglu, R, Ezzat, K, Stangl, H, Plochberger, B, Laurencikiene, J, El Andaloussi, S, Fürth, D, Stevens, MM & Sezgin, E 2024, 'High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler', Nature Biotechnology, vol. 42, no. 4, pp. 587-590. https://doi.org/10.1038/s41587-023-01825-5

TY - JOUR

T1 - High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler

AU - Sych, Taras

AU - Schlegel, Jan

AU - Barriga, Hanna M.G.

AU - Ojansivu, Miina

AU - Hanke, Leo

AU - Weber, Florian

AU - Beklem Bostancioglu, R.

AU - Ezzat, Kariem

AU - Stangl, Herbert

AU - Plochberger, Birgit

AU - Laurencikiene, Jurga

AU - El Andaloussi, Samir

AU - Fürth, Daniel

AU - Stevens, Molly M.

AU - Sezgin, Erdinc

PY - 2024/4

Y1 - 2024/4

N2 - We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5–200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.

AB - We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5–200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.

KW - Particle Size

KW - Liposomes/chemistry

KW - Nanoparticles/chemistry

UR - https://www.scopus.com/pages/publications/85163136152

U2 - 10.1038/s41587-023-01825-5

DO - 10.1038/s41587-023-01825-5

M3 - Article

C2 - 37308687

AN - SCOPUS:85163136152

SN - 1087-0156

VL - 42

SP - 587

EP - 590

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 4

ER -

High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler

Abstract

Keywords

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