TY - JOUR
T1 - “Head-to-Toe” Lipid Properties Govern the Binding and Cargo Transfer of High-Density Lipoprotein
AU - Weber, Florian
AU - Axmann, Markus
AU - Sezgin, Erdinc
AU - Amaro, Mariana
AU - Sych, Taras
AU - Hochreiner, Armin
AU - Hof, Martin
AU - Schütz, Gerhard J.
AU - Stangl, Herbert
AU - Plochberger, Birgit
N1 - Publisher Copyright:
© 2024 by the authors.
PY - 2024/12/6
Y1 - 2024/12/6
N2 - The viscoelastic properties of biological membranes are crucial in controlling cellular functions and are determined primarily by the lipids’ composition and structure. This work studies these properties by varying the structure of the constituting lipids in order to influence their interaction with high-density lipoprotein (HDL) particles. Various fluorescence-based techniques were applied to study lipid domains, membrane order, and the overall lateral as well as the molecule–internal glycerol region mobility in HDL–membrane interactions (i.e., binding and/or cargo transfer). The analysis of interactions with HDL particles and various lipid phases revealed that both fully fluid and some gel-phase lipids preferentially interact with HDL particles, although differences were observed in protein binding and cargo exchange. Both interactions were reduced with ordered lipid mixtures containing cholesterol. To investigate the mechanism, membranes were prepared from single-lipid components, enabling step-by-step modification of the lipid building blocks. On a biophysical level, the different mixtures displayed varying stiffness, fluidity, and hydrogen bond network changes. Increased glycerol mobility and a strengthened hydrogen bond network enhanced anchoring interactions, while fluid membranes with a reduced water network facilitated cargo transfer. In summary, the data indicate that different lipid classes are involved depending on the type of interaction, whether anchoring or cargo transfer.
AB - The viscoelastic properties of biological membranes are crucial in controlling cellular functions and are determined primarily by the lipids’ composition and structure. This work studies these properties by varying the structure of the constituting lipids in order to influence their interaction with high-density lipoprotein (HDL) particles. Various fluorescence-based techniques were applied to study lipid domains, membrane order, and the overall lateral as well as the molecule–internal glycerol region mobility in HDL–membrane interactions (i.e., binding and/or cargo transfer). The analysis of interactions with HDL particles and various lipid phases revealed that both fully fluid and some gel-phase lipids preferentially interact with HDL particles, although differences were observed in protein binding and cargo exchange. Both interactions were reduced with ordered lipid mixtures containing cholesterol. To investigate the mechanism, membranes were prepared from single-lipid components, enabling step-by-step modification of the lipid building blocks. On a biophysical level, the different mixtures displayed varying stiffness, fluidity, and hydrogen bond network changes. Increased glycerol mobility and a strengthened hydrogen bond network enhanced anchoring interactions, while fluid membranes with a reduced water network facilitated cargo transfer. In summary, the data indicate that different lipid classes are involved depending on the type of interaction, whether anchoring or cargo transfer.
KW - Laurdan polarity
KW - glycerol region mobility
KW - hydrogen bond network
KW - lipoprotein
KW - membrane order
UR - http://www.scopus.com/inward/record.url?scp=85213417256&partnerID=8YFLogxK
U2 - 10.3390/membranes14120261
DO - 10.3390/membranes14120261
M3 - Article
C2 - 39728711
SN - 2077-0375
VL - 14
JO - Membranes
JF - Membranes
IS - 12
M1 - 261
ER -