TY - JOUR
T1 - Grape seed extract improves intestinal barrier integrity and performance
T2 - Evidence from in vitro, Caenorhabditis elegans and Drosophila melanogaster experiments and a study with growing broilers
AU - Sandner, Georg
AU - Stadlbauer, Verena
AU - Sadova, Nadiia
AU - Neuhauser, Cathrina
AU - Schwarzinger, Bettina
AU - Karlsberger, Lea
AU - Hangweirer, Kerstin
AU - Antensteiner, Katharina
AU - Stallinger, Alexander
AU - Aumiller, Tobias
AU - Weghuber, Julian
N1 - Funding Information:
This work was created within a research project of the Austrian Competence Centre for Feed and Food Quality, Safety and Innovation (FFoQSI). The COMET-K1 Competence Centre FFoQSI is funded by the Austrian ministries BMVIT, BMDW and the Austrian provinces Lower Austria, Upper Austria and Vienna within the scope of COMET-Competence Centers for Excellent Technologies. The programme COMET is handled by the Austrian Research Promotion Agency FFG (grant 881882). This research was also funded by the Christian Doppler Forschungsgesellschaft (Josef Ressel Centre for Phytogenic Drug Research) and the ‘Dissertationsprogramm der Fachhochschule OÖ 2020’ with the financial support of the province of Upper Austria (FFG grant 868740).
Funding Information:
This work was created within a research project of the Austrian Competence Centre for Feed and Food Quality, Safety and Innovation (FFoQSI). The COMET-K1 Competence Centre FFoQSI is funded by the Austrian ministries BMVIT , BMDW and the Austrian provinces Lower Austria, Upper Austria and Vienna within the scope of COMET-Competence Centers for Excellent Technologies . The programme COMET is handled by the Austrian Research Promotion Agency FFG (grant 881882 ). This research was also funded by the Christian Doppler Forschungsgesellschaft (Josef Ressel Centre for Phytogenic Drug Research) and the ‘ Dissertationsprogramm der Fachhochschule OÖ 2020 ’ with the financial support of the province of Upper Austria (FFG grant 868740 ).
Publisher Copyright:
© 2023 The Author(s)
PY - 2023/4
Y1 - 2023/4
N2 - Grape seed extract (GSE) is an interesting source for polyphenols and other phytochemicals with high antioxidant potential. Here, we report on a comprehensive study investigating GSE in various in vitro and in vivo experiments. Obtained results indicate that GSE improves parameters related to intestinal barrier integrity in cell culture, C. elegans and D. melanogaster. Faster wound healing was observed in a scratch assay using intestinal IPEC-J2 cells when pre-treated with GSE. Furthermore, reactive oxygen species were found to be significantly decreased in cell culture and C. elegans experiments. In C. elegans, qPCR gene expression analysis revealed significant modulations after 24 h of GSE treatment. Finally, a 42-day broiler feeding study revealed a dose dependant effect on growth performance in the GSE supplemented groups. In conclusion, GSE was found to provide promising properties including wound closure, intestinal integrity, and antioxidant activity.
AB - Grape seed extract (GSE) is an interesting source for polyphenols and other phytochemicals with high antioxidant potential. Here, we report on a comprehensive study investigating GSE in various in vitro and in vivo experiments. Obtained results indicate that GSE improves parameters related to intestinal barrier integrity in cell culture, C. elegans and D. melanogaster. Faster wound healing was observed in a scratch assay using intestinal IPEC-J2 cells when pre-treated with GSE. Furthermore, reactive oxygen species were found to be significantly decreased in cell culture and C. elegans experiments. In C. elegans, qPCR gene expression analysis revealed significant modulations after 24 h of GSE treatment. Finally, a 42-day broiler feeding study revealed a dose dependant effect on growth performance in the GSE supplemented groups. In conclusion, GSE was found to provide promising properties including wound closure, intestinal integrity, and antioxidant activity.
KW - Broiler
KW - C. elegans
KW - D. melanogaster
KW - Grape seed extract
KW - Intestinal barrier
KW - Intestinal functionality
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=85148335248&partnerID=8YFLogxK
U2 - 10.1016/j.fbio.2023.102483
DO - 10.1016/j.fbio.2023.102483
M3 - Article
AN - SCOPUS:85148335248
SN - 2212-4292
VL - 52
JO - Food Bioscience
JF - Food Bioscience
M1 - 102483
ER -