Ginseng Extract Ameliorates the Negative Physiological Effects of Heat Stress by Supporting Heat Shock Response and Improving Intestinal Barrier Integrity: Evidence from Studies with Heat-Stressed Caco-2 Cells, C. elegans and Growing Broilers

Georg Philipp Sandner, Andreas Müller, Xiaodan Zhou, Verena Stadlbauer, Bettina Schwarzinger, Clemens Schwarzinger, Klaus Männer, Uwe Wenzel, Tobias Aumiller, Julian Weghuber

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Climatic changes and heat stress have become a great challenge in the livestock industry, negatively affecting, in particular, poultry feed intake and intestinal barrier malfunction. Recently, phytogenic feed additives were applied to reduce heat stress effects on animal farming. Here, we investigated the effects of ginseng extract using various in vitro and in vivo experiments. Quantitative real-time PCR, transepithelial electrical resistance measurements and survival assays under heat stress conditions were carried out in various model systems, including Caco-2 cells, Caenorhabditis elegans and jejunum samples of broilers. Under heat stress conditions, ginseng treatment lowered the expression of HSPA1A (Caco-2) and the heat shock protein genes hsp-1 and hsp-16.2 (both in C. elegans), while all three of the tested genes encoding tight junction proteins, CLDN3, OCLN and CLDN1 (Caco-2), were upregulated. In addition, we observed prolonged survival under heat stress in Caenorhabditis elegans, and a better performance of growing ginseng-fed broilers by the increased gene expression of selected heat shock and tight junction proteins. The presence of ginseng extract resulted in a reduced decrease in transepithelial resistance under heat shock conditions. Finally, LC-MS analysis was performed to quantitate the most prominent ginsenosides in the extract used for this study, being Re, Rg1, Rc, Rb2 and Rd. In conclusion, ginseng extract was found to be a suitable feed additive in animal nutrition to reduce the negative physiological effects caused by heat stress.
Original languageEnglish
Article number835
JournalMolecules
Volume25
Issue number4
DOIs
Publication statusPublished - 14 Feb 2020

Keywords

  • Broiler
  • Ginseng extract
  • Heat stress
  • Intestinal barrier
  • Caco-2 Cells
  • Panax/chemistry
  • Caenorhabditis elegans/drug effects
  • Humans
  • Claudin-1/genetics
  • Animals
  • Chickens
  • HSP70 Heat-Shock Proteins/genetics
  • Heat Stress Disorders/drug therapy
  • Heat-Shock Response/drug effects
  • Jejunum/drug effects
  • Plant Extracts/chemistry
  • Caenorhabditis elegans Proteins/genetics
  • Gene Expression Regulation/drug effects
  • Claudin-3/genetics

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