Frequency-domain photoacoustic and fluorescence microscopy: Application on labeled and unlabeled cells

Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Karoline Pfeffer, Sven Wohlfarth, Günther Hannesschläger, Thomas A. Klar, Thomas Berer

Research output: Chapter in Book/Report/Conference proceedingsConference contributionpeer-review

2 Citations (Scopus)


In this paper, multimodal optical-resolution frequency-domain photoacoustic and fluorescence scanning microscopy is presented on labeled and unlabeled cells. In many molecules, excited electrons relax radiatively and non-radiatively, leading to fluorescence and photoacoustic signals, respectively. Both signals can then be detected simultaneously. There also exist molecules, e.g. hemoglobin, which do not exhibit fluorescence, but provide photoacoustic signals solely. Other molecules, especially fluorescent dyes, preferentially exhibit fluorescence. The fluorescence quantum yield of a molecule and with it the strength of photoacoustic and fluorescence signals depends on the local environment, e.g. on the pH. Therefore, the local distribution of the simultaneously recorded photoacoustic and fluorescence signals may be used in order to obtain information about the local chemistry.

Original languageEnglish
Title of host publicationMultimodal Biomedical Imaging XIII
EditorsFred S. Azar, Xavier Intes
ISBN (Electronic)9781510614598
Publication statusPublished - 2018
EventMultimodal Biomedical Imaging XIII 2018 - San Francisco, United States
Duration: 27 Jan 201828 Jan 2018

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceMultimodal Biomedical Imaging XIII 2018
Country/TerritoryUnited States
CitySan Francisco


  • fluorescence microscopy
  • frequency domain
  • multimodal microscopy
  • photoacoustic microscopy


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