Fabrication, characterization and application of biomolecule micropatterns on cyclic olefin polymer (COP) surfaces with adjustable contrast

Roland Hager, Thomas Haselgrübler, Sandra Haas, Anna Maria Lipp, Julian Weghuber

Research output: Contribution to journalArticlepeer-review

Abstract

Peptide and protein micropatterns are powerful tools for the investigation of various cellular processes, including protein-protein interactions (PPIs). Within recent years, various approaches for the production of functional surfaces have been developed. Most of these systems use glass as a substrate, which has several drawbacks, including high fragility and costs, especially if implemented for fluorescence microscopy. In addition, conventional fabrication technologies such as microcontact printing (µCP) are frequently used for the transfer of biomolecules to the glass surface. In this case, it is challenging to adjust the biomolecule density. Here, we show that cyclic olefin polymer (COP) foils, with their encouraging properties, including the ease of manufacturing, chemical resistance, biocompatibility, low water absorption, and optical clarity, are a promising alternative to glass substrates for the fabrication of micropatterns. Using a photolithography-based approach, we generated streptavidin/biotinylated antibody patterns on COPs with the possibility of adjusting the pattern contrast by varying plasma activation parameters. Our experimental setup was finally successfully implemented for the analysis of PPIs in the membranes of live cells via total internal reflection fluorescence (TIRF) microscopy.

Original languageEnglish
Article number3
JournalBiosensors
Volume10
Issue number1
DOIs
Publication statusPublished - 28 Dec 2019

Keywords

  • Cyclic olefin polymer
  • Micropatterned polymers
  • Micropatterns
  • Photolithography
  • Protein-protein interaction
  • Total internal reflection fluorescence (TIRF)
  • Optical Imaging
  • Jurkat Cells
  • Humans
  • Cells, Cultured
  • Cycloparaffins/chemistry
  • Polymers/chemistry
  • Surface Properties
  • Biosensing Techniques
  • Microscopy, Fluorescence

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