Effects of Progesterone and its Metabolites on Human Granulosa Cells: Progesterone and Granulosa Cells

Detlef Pietrowski, Yao Gong, Mario Mairhofer, R. Gessele, Michael Sator

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The corpus luteum (CL) is under control of gonadotrophic hormones and produces progesterone, which is necessary for endometrial receptivity. Recent studies have shown that progesterone and its metabolites are involved in cell proliferation and apoptosis of cancer cells. Here weanalyzed the role of progesterone and its meta­bolites on luteinized granulosa cells (LGC) by FACS analysis and quantitative Real-Time PCR. We detected the mRNA of the progesterone metabolizing genes SRD5A1, AKR1C1, and AKR1C2 in LGC. The stimulation of LGC with progesterone or progesterone metabolites did not show any effect on the mRNA expression of these genes. However, a downregulation of Fas expression was found to be accomplished by progesterone and human chorionic gonadotropin. Our findings do not support the concept of an effect of progesterone metabolites on LGCs. However, it suggests an antiapoptotic effect of hCG and progesterone during corpus luteum development by downregulation of Fas.
Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalHormone and Metabolic Research
Volume46
Issue number2
DOIs
Publication statusPublished - Feb 2014

Keywords

  • chorionic gonadotropin - apoptosis - in vitro fertilization - corpus luteum
  • in vitro fertilization
  • apoptosis
  • corpus luteum
  • chorionic gonadotropin

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