TY - JOUR
T1 - Effects of Progesterone and its Metabolites on Human Granulosa Cells
T2 - Progesterone and Granulosa Cells
AU - Pietrowski, Detlef
AU - Gong, Yao
AU - Mairhofer, Mario
AU - Gessele, R.
AU - Sator, Michael
PY - 2014/2
Y1 - 2014/2
N2 - The corpus luteum (CL) is under control of gonadotrophic hormones and produces progesterone, which is necessary for endometrial receptivity. Recent studies have shown that progesterone and its metabolites are involved in cell proliferation and apoptosis of cancer cells. Here weanalyzed the role of progesterone and its metabolites on luteinized granulosa cells (LGC) by FACS analysis and quantitative Real-Time PCR. We detected the mRNA of the progesterone metabolizing genes SRD5A1, AKR1C1, and AKR1C2 in LGC. The stimulation of LGC with progesterone or progesterone metabolites did not show any effect on the mRNA expression of these genes. However, a downregulation of Fas expression was found to be accomplished by progesterone and human chorionic gonadotropin. Our findings do not support the concept of an effect of progesterone metabolites on LGCs. However, it suggests an antiapoptotic effect of hCG and progesterone during corpus luteum development by downregulation of Fas.
AB - The corpus luteum (CL) is under control of gonadotrophic hormones and produces progesterone, which is necessary for endometrial receptivity. Recent studies have shown that progesterone and its metabolites are involved in cell proliferation and apoptosis of cancer cells. Here weanalyzed the role of progesterone and its metabolites on luteinized granulosa cells (LGC) by FACS analysis and quantitative Real-Time PCR. We detected the mRNA of the progesterone metabolizing genes SRD5A1, AKR1C1, and AKR1C2 in LGC. The stimulation of LGC with progesterone or progesterone metabolites did not show any effect on the mRNA expression of these genes. However, a downregulation of Fas expression was found to be accomplished by progesterone and human chorionic gonadotropin. Our findings do not support the concept of an effect of progesterone metabolites on LGCs. However, it suggests an antiapoptotic effect of hCG and progesterone during corpus luteum development by downregulation of Fas.
KW - chorionic gonadotropin - apoptosis - in vitro fertilization - corpus luteum
KW - chorionic gonadotropin - apoptosis - in vitro fertilization - corpus luteum
KW - in vitro fertilization
KW - apoptosis
KW - corpus luteum
KW - chorionic gonadotropin
UR - http://www.scopus.com/inward/record.url?scp=84894263303&partnerID=8YFLogxK
U2 - 10.1055/s-0033-1357167
DO - 10.1055/s-0033-1357167
M3 - Article
SN - 0018-5043
VL - 46
SP - 133
EP - 137
JO - Hormone and Metabolic Research
JF - Hormone and Metabolic Research
IS - 2
ER -
