TY - JOUR
T1 - Different types of cell-to-cell connections mediated by nanotubular structures
AU - Veranič, Peter
AU - Lokar, Maruša
AU - Schütz, Gerhard J.
AU - Weghuber, Julian
AU - Wieser, Stefan
AU - Hägerstrand, Henry
AU - Kralj-Iglič, Veronika
AU - Iglič, Aleš
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2008/11/1
Y1 - 2008/11/1
N2 - Communication between cells is crucial for proper functioning of multicellular organisms. The recently discovered membranous tubes, named tunneling nanotubes, that directly bridge neighboring cells may offer a very specific and effective way of intercellular communication. Our experiments on RT4 and T24 urothelial cell lines show that nanotubes that bridge neighboring cells can be divided into two types. The nanotubes of type I are shorter and more dynamic than those of type II, and they contain actin filaments. They are formed when cells explore their surroundings to make contact with another cell. The nanotubes of type II are longer and more stable than type I, and they have cytokeratin filaments. They are formed when two already connected cells start to move apart. On the nanotubes of both types, small vesicles were found as an integral part of the nanotubes (that is, dilatations of the nanotubes). The dilatations of type II nanotubes do not move along the nanotubes, whereas the nanotubes of type I frequently have dilatations (gondolas) that move along the nanotubes in both directions. A possible model of formation and mechanical stability of nanotubes that bridge two neighboring cells is discussed.
AB - Communication between cells is crucial for proper functioning of multicellular organisms. The recently discovered membranous tubes, named tunneling nanotubes, that directly bridge neighboring cells may offer a very specific and effective way of intercellular communication. Our experiments on RT4 and T24 urothelial cell lines show that nanotubes that bridge neighboring cells can be divided into two types. The nanotubes of type I are shorter and more dynamic than those of type II, and they contain actin filaments. They are formed when cells explore their surroundings to make contact with another cell. The nanotubes of type II are longer and more stable than type I, and they have cytokeratin filaments. They are formed when two already connected cells start to move apart. On the nanotubes of both types, small vesicles were found as an integral part of the nanotubes (that is, dilatations of the nanotubes). The dilatations of type II nanotubes do not move along the nanotubes, whereas the nanotubes of type I frequently have dilatations (gondolas) that move along the nanotubes in both directions. A possible model of formation and mechanical stability of nanotubes that bridge two neighboring cells is discussed.
KW - Animals
KW - Biochemical Phenomena
KW - Cattle
KW - Cell Communication
KW - Cell Line
KW - Cell Surface Extensions/metabolism
KW - Epithelial Cells/cytology
KW - Humans
KW - Mice
KW - Microscopy, Electron, Transmission
KW - Microscopy, Fluorescence
KW - Microscopy, Phase-Contrast
KW - Models, Biological
KW - Tomography, X-Ray Computed
KW - Urinary Bladder/cytology
UR - http://www.scopus.com/inward/record.url?scp=58149160376&partnerID=8YFLogxK
U2 - 10.1529/biophysj.108.131375
DO - 10.1529/biophysj.108.131375
M3 - Article
C2 - 18658210
SN - 0006-3495
VL - 95
SP - 4416
EP - 4425
JO - Biophysical Journal
JF - Biophysical Journal
IS - 9
ER -