Combining TIR and FRET: From fluorescence microscopy to a multi-well reader system

Herbert Schneckenburger, Petra Weber, Michael Wagner, Sandra Enderle, Julian Weghuber, Peter Lanzerstorfer

Research output: Chapter in Book/Report/Conference proceedingsConference contributionpeer-review

1 Citation (Scopus)


In view of a pharmacological test system with living cells we examined Förster Resonance Energy Transfer (FRET) between fluorescent proteins upon Total Internal Reflection (TIR) of a laser beam. We measured in particular HeLa cells expressing membrane associated EGFR-CFP and Grb2-YFP prior to and subsequent to stimulation with the epidermal growth factor EGF. In addition, HeLa cells expressing a membrane associated Green Fluorescent Protein (HeLa hFR-GPI-GFP) were incubated with the energy acceptor Nile Red for further validation. Measurements range from fluorescence microscopy to Fluorescence Lifetime Imaging (FLIM) of a larger number of samples in a TIR reader.

Original languageEnglish
Title of host publicationAdvances in Microscopic Imaging II
EditorsEmmanuel Beaurepaire, Francesco Saverio Pavone
ISBN (Electronic)9781510628458
Publication statusPublished - 2019
EventAdvances in Microscopic Imaging II 2019 - Munich, Germany
Duration: 26 Jun 201927 Jun 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceAdvances in Microscopic Imaging II 2019


  • FLIM
  • Fluorescence microscopy
  • Fluorescence reader
  • FRET
  • Living cells
  • TIR


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