Coelastrella terrestris for Adonixanthin Production: Physiological Characterization and Evaluation of Secondary Carotenoid Productivity

Philipp Doppler, Ricarda Kriechbaum, Maria Käfer, Julian Kopp, Daniel Remias, Oliver Spadiut

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A novel strain of Coelastrella terrestris (Chlorophyta) was collected from red mucilage in a glacier foreland in Iceland. Its morphology showed characteristic single, ellipsoidal cells with apical wart-like wall thickenings. Physiological characterization revealed the presence of the rare keto-carotenoid adonixanthin, as well as high levels of unsaturated fatty acids of up to 85%. Initial screening experiments with different carbon sources for accelerated mixotrophic biomass growth were done. Consequently, a scale up to 1.25 L stirred photobioreactor cultivations yielded a maximum of 1.96 mg·L−1 adonixanthin in free and esterified forms. It could be shown that supplementing acetate to the medium increased the volumetric productivity after entering the nitrogen limitation phase compared to autotrophic control cultures. This study describes a promising way of biotechnological adonixanthin production using Coelastrella terrestris.

Original languageEnglish
Article number175
JournalMarine Drugs
Volume20
Issue number3
DOIs
Publication statusPublished - Mar 2022

Keywords

  • Astaxanthin
  • Canthaxanthin
  • Microalgae
  • Nutrient starvation
  • Osmotic stress
  • Stirred photobioreactor
  • Unsaturated fatty acids
  • Fatty Acids/metabolism
  • Microalgae/growth & development
  • Chlorophyceae/growth & development
  • Photobioreactors
  • Nitrates/analysis
  • Carotenoids/metabolism
  • Acetates/metabolism

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