Abstract
While long-term effects of temperature treatment in respect of, e.g., gene-expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short-term hypothermal shifts. In this report, we characterized the effects of such a stimulation using the human lymphocyte cell line Jurkat E6.1 and present evidence that warming from 4 to 37 degrees C for only 2 min is sufficient to cause co-localization of CD3, prion protein and the lipid-raft ganglioside GM1 paralleling lymphocyte activation as observed by Ca(2+) mobilization and mitogen-activated protein kinase-phosphorylation.
| Original language | English |
|---|---|
| Pages (from-to) | 121-125 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 566 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - 21 May 2004 |
| Externally published | Yes |
Keywords
- CT-B, cholera toxin subunit B
- GPI, glycosylphosphatidylinositol
- Lymphocyte
- MβCD, methyl-β-cyclodextrin
- MAPK, mitogen-activated protein kinase
- PrP, prion protein
- SAPK, stress-activated protein kinase
- T-cell receptor
- TCR, T-cell receptor
- Actins/metabolism
- Humans
- T-Lymphocytes/metabolism
- CD3 Complex/chemistry
- Calcium/chemistry
- Protein Structure, Tertiary
- Jurkat Cells
- Lymphocyte Activation
- Cyclodextrins/pharmacology
- Cytochalasin D/pharmacology
- beta-Cyclodextrins
- Cell Membrane/metabolism
- Mitogen-Activated Protein Kinases/metabolism
- Prions/metabolism
- Fluorescent Antibody Technique
- Hypothermia, Induced
- Enzyme Activation
- Cholesterol/deficiency
- G(M1) Ganglioside/chemistry
