Co-localization of CD3 and prion protein in Jurkat lymphocytes after hypothermal stimulation

Susanne Wurm, Christian Paar, Alois Sonnleitner, Max Sonnleitner, Otmar Höglinger, Christoph Romanin, Christian Wechselberger

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


While long-term effects of temperature treatment in respect of, e.g., gene-expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short-term hypothermal shifts. In this report, we characterized the effects of such a stimulation using the human lymphocyte cell line Jurkat E6.1 and present evidence that warming from 4 to 37 degrees C for only 2 min is sufficient to cause co-localization of CD3, prion protein and the lipid-raft ganglioside GM1 paralleling lymphocyte activation as observed by Ca(2+) mobilization and mitogen-activated protein kinase-phosphorylation.

Original languageEnglish
Pages (from-to)121-125
Number of pages5
JournalFEBS Letters
Issue number1-3
Publication statusPublished - 21 May 2004
Externally publishedYes


  • CT-B, cholera toxin subunit B
  • GPI, glycosylphosphatidylinositol
  • Lymphocyte
  • MβCD, methyl-β-cyclodextrin
  • MAPK, mitogen-activated protein kinase
  • PrP, prion protein
  • SAPK, stress-activated protein kinase
  • T-cell receptor
  • TCR, T-cell receptor
  • Actins/metabolism
  • Humans
  • T-Lymphocytes/metabolism
  • CD3 Complex/chemistry
  • Calcium/chemistry
  • Protein Structure, Tertiary
  • Jurkat Cells
  • Lymphocyte Activation
  • Cyclodextrins/pharmacology
  • Cytochalasin D/pharmacology
  • beta-Cyclodextrins
  • Cell Membrane/metabolism
  • Mitogen-Activated Protein Kinases/metabolism
  • Prions/metabolism
  • Fluorescent Antibody Technique
  • Hypothermia, Induced
  • Enzyme Activation
  • Cholesterol/deficiency
  • G(M1) Ganglioside/chemistry


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