Cholesterol Slows down the Lateral Mobility of an Oxidized Phospholipid in a Supported Lipid Bilayer

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25 Citations (Scopus)

Abstract

We investigated the mobility and phase-partitioning of the fluorescent oxidized phospholipid analogue 1-palmitoyl-2-glutaroyl-sn-glycero-3-phospho-N- Alexa647-ethanolamine (PGPE-Alexa647) in supported lipid bilayers. Compared to the conventional phospholipid dihexadecanoylphosphoethanolamine (DHPE)-Bodipy we found consistently higher diffusion constants. The effect became dramatic when immobile obstacles were inserted into the bilayer, which essentially blocked the diffusion of DHPE-Bodipy but hardly influenced the movements of PGPE-Alexa647. In a supported lipid bilayer made of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), the differences in probe mobility leveled off with increasing cholesterol content. Using coarse-grained molecular dynamics simulations, we could ascribe this effect to increased interactions between the oxidized phospholipid and the membrane matrix, concomitant with a translation in the headgroup position of the oxidized phospholipid: at zero cholesterol content, its headgroup is shifted to the outside of the DOPC headgroup region, whereas increasing cholesterol concentrations pulls the headgroup into the bilayer plane.

Original languageEnglish
Pages (from-to)17322–17329
Number of pages8
JournalLangmuir
Volume26
Issue number22
DOIs
Publication statusPublished - 16 Nov 2010

Keywords

  • Cholesterol/chemistry
  • Diffusion
  • Ethanolamines/chemistry
  • Kinetics
  • Lipid Bilayers/chemistry
  • Molecular Conformation
  • Molecular Dynamics Simulation
  • Movement
  • Oxidation-Reduction
  • Phospholipids/chemistry

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