Abstract
Mesotaenium berggrenii is one of few autotrophs that thrive on bare glacier surfaces in alpine and polar regions. This extremophilic alga produces high amounts of a brownish vacuolar pigment, whose chemical constitution and ecological function is largely unknown until now. Field material was harvested to isolate and characterize this pigment. Its tannin nature was determined by photometric methods, and the structure determination was carried out by means of HPLC-MS and 1D- and 2D-NMR spectroscopy. The main constituent turned out to be purpurogallin carboxylic acid-6-O-β-d-glucopyranoside. This is the first report of such a phenolic compound in this group of algae. Because of its broad absorption capacities of harmful UV and excessive VIS radiation, this secondary metabolite seems to play an important role for the survival of this alga at exposed sites. Attributes and abundances of the purpurogallins found in M. berggrenii strongly suggest that they are of principal ecophysiological relevance like analogous protective pigments of other extremophilic microorganisms. To prove that M. berggrenii is a true psychrophile, photosynthesis measurements at ambient conditions were carried out. Sequencing of the 18S rRNA gene of this alpine species and of its arctic relative, the filamentous Ancylonema nordenskiöldii, underlined their distinct taxonomic position within the Zygnematophyceae.
Original language | English |
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Pages (from-to) | 638-648 |
Number of pages | 11 |
Journal | FEMS Microbiology Ecology |
Volume | 79 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 2012 |
Externally published | Yes |
Keywords
- European Alps
- Life on ice
- Phenolic pigment
- Psychrophile
- UV radiation
- Photosynthesis/physiology
- Absorption
- Ultraviolet Rays
- Benzocycloheptenes/chemistry
- Arctic Regions
- Streptophyta/genetics
- Ice Cover