Cell surface localised Hsp70 is a cancer specific regulator of clathrin-independent endocytosis

Benedikt Nimmervoll; Lilia Chtcheglova; Kata Juhasz; N Cremades; F.A. Aprile; Alois Sonnleitner; Peter Hinterdorfer; L. Vigh; Johannes Preiner; Zolt Balogi

doi:10.1016/j.febslet.2015.07.037

Cell surface localised Hsp70 is a cancer specific regulator of clathrin-independent endocytosis

Benedikt Nimmervoll, Lilia Chtcheglova, Kata Juhasz, N Cremades, F.A. Aprile, Alois Sonnleitner, Peter Hinterdorfer, L. Vigh, Johannes Preiner, Zolt Balogi

Research output: Contribution to journal › Article

42 Citations (Scopus)

Abstract

The stress inducible heat shock protein 70 (Hsp70) is present specifically on the tumour cell surface yet without a pro-tumour function revealed. We show here that cell surface localised Hsp70 (sHsp70) supports clathrin-independent endocytosis (CIE) in melanoma models. Remarkably, ability of Hsp70 to cluster on lipid rafts in vitro correlated with larger nano-domain sizes of sHsp70 in high sHsp70 expressing cell membranes. Interfering with Hsp70 oligomerisation impaired sHsp70-mediated facilitation of endocytosis. Altogether our findings suggest that a sub-fraction of sHsp70 co-localising with lipid rafts enhances CIE through oligomerisation and clustering. Targeting or utilising this tumour specific mechanism may represent an additional benefit for anti-cancer therapy.

Original language	English
Pages (from-to)	2747-2753
Number of pages	7
Journal	FEBS Letters
Volume	589
Issue number	19
DOIs	https://doi.org/10.1016/j.febslet.2015.07.037
Publication status	Published - 14 Sept 2015

Keywords

Cancer
Clustering
Endocytosis
Hsp70
Membrane

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.febslet.2015.07.037

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title = "Cell surface localised Hsp70 is a cancer specific regulator of clathrin-independent endocytosis",

abstract = "The stress inducible heat shock protein 70 (Hsp70) is present specifically on the tumour cell surface yet without a pro-tumour function revealed. We show here that cell surface localised Hsp70 (sHsp70) supports clathrin-independent endocytosis (CIE) in melanoma models. Remarkably, ability of Hsp70 to cluster on lipid rafts in vitro correlated with larger nano-domain sizes of sHsp70 in high sHsp70 expressing cell membranes. Interfering with Hsp70 oligomerisation impaired sHsp70-mediated facilitation of endocytosis. Altogether our findings suggest that a sub-fraction of sHsp70 co-localising with lipid rafts enhances CIE through oligomerisation and clustering. Targeting or utilising this tumour specific mechanism may represent an additional benefit for anti-cancer therapy.",

keywords = "Cancer, Clustering, Endocytosis, Hsp70, Membrane",

author = "Benedikt Nimmervoll and Lilia Chtcheglova and Kata Juhasz and N Cremades and F.A. Aprile and Alois Sonnleitner and Peter Hinterdorfer and L. Vigh and Johannes Preiner and Zolt Balogi",

note = "Funding Information: This work was funded by the European Regional Development Fund (EFRE) , the state of Upper Austria. Laszlo Vigh was supported by the Hungarian Basic Research Fund (OTKA, No. 100857). Nunilo Cremades is a Royal Society Dorothy Hodgkin Research Fellow. Publisher Copyright: {\textcopyright} 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2015",

month = sep,

day = "14",

doi = "10.1016/j.febslet.2015.07.037",

language = "English",

volume = "589",

pages = "2747--2753",

journal = "FEBS Letters",

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T1 - Cell surface localised Hsp70 is a cancer specific regulator of clathrin-independent endocytosis

AU - Nimmervoll, Benedikt

AU - Chtcheglova, Lilia

AU - Juhasz, Kata

AU - Cremades, N

AU - Aprile, F.A.

AU - Sonnleitner, Alois

AU - Hinterdorfer, Peter

AU - Vigh, L.

AU - Preiner, Johannes

AU - Balogi, Zolt

N1 - Funding Information: This work was funded by the European Regional Development Fund (EFRE) , the state of Upper Austria. Laszlo Vigh was supported by the Hungarian Basic Research Fund (OTKA, No. 100857). Nunilo Cremades is a Royal Society Dorothy Hodgkin Research Fellow. Publisher Copyright: © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2015/9/14

Y1 - 2015/9/14

N2 - The stress inducible heat shock protein 70 (Hsp70) is present specifically on the tumour cell surface yet without a pro-tumour function revealed. We show here that cell surface localised Hsp70 (sHsp70) supports clathrin-independent endocytosis (CIE) in melanoma models. Remarkably, ability of Hsp70 to cluster on lipid rafts in vitro correlated with larger nano-domain sizes of sHsp70 in high sHsp70 expressing cell membranes. Interfering with Hsp70 oligomerisation impaired sHsp70-mediated facilitation of endocytosis. Altogether our findings suggest that a sub-fraction of sHsp70 co-localising with lipid rafts enhances CIE through oligomerisation and clustering. Targeting or utilising this tumour specific mechanism may represent an additional benefit for anti-cancer therapy.

AB - The stress inducible heat shock protein 70 (Hsp70) is present specifically on the tumour cell surface yet without a pro-tumour function revealed. We show here that cell surface localised Hsp70 (sHsp70) supports clathrin-independent endocytosis (CIE) in melanoma models. Remarkably, ability of Hsp70 to cluster on lipid rafts in vitro correlated with larger nano-domain sizes of sHsp70 in high sHsp70 expressing cell membranes. Interfering with Hsp70 oligomerisation impaired sHsp70-mediated facilitation of endocytosis. Altogether our findings suggest that a sub-fraction of sHsp70 co-localising with lipid rafts enhances CIE through oligomerisation and clustering. Targeting or utilising this tumour specific mechanism may represent an additional benefit for anti-cancer therapy.

KW - Cancer

KW - Clustering

KW - Endocytosis

KW - Hsp70

KW - Membrane

UR - https://www.scopus.com/pages/publications/84941420549

U2 - 10.1016/j.febslet.2015.07.037

DO - 10.1016/j.febslet.2015.07.037

M3 - Article

SN - 0014-5793

VL - 589

SP - 2747

EP - 2753

JO - FEBS Letters

JF - FEBS Letters

IS - 19

ER -

Cell surface localised Hsp70 is a cancer specific regulator of clathrin-independent endocytosis

Abstract

Keywords

UN SDGs

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