Biomedical Sensing with the Atomic Force Microscope

Constanze Lamprecht, Jürgen Strasser, Melanie Köhler, Sandra Posch, Yoo Jin Oh, Rong Zhu, Lilia Chtcheglova, Andreas Ebner, Peter Hinterdorfer

Research output: Chapter in Book/Report/Conference proceedingsChapter

1 Citation (Scopus)

Abstract

In this chapter we highlight the use and advantages of the atomic force microscope (AFM) in life science. Our aim is to present the wealth of experimental possibilities provided by this powerful toolbox with special regard to biomedical sensing applications. Originally invented in the 1980s to visualize solid surfaces on the nanometer scale, today AFM imaging is routinely used to nondestructively map the surface-ultrastructure of soft biological samples under physiological conditions with unprecedented lateral resolution. Owing to its force detection sensitivity that ranges from nano-Newton down to a few pico-Newton the AFM has become an established technique for exploring kinetic and structural details of inter- and intramolecular interactions and biomolecular recognition processes. The combination of such single molecule force measurements with topographical imaging has led to the development of recognition imaging, which allows for identification and mapping of specific components in complex biological samples with high spatial accuracy. In the following, the basic principles of biologically relevant AFM imaging modes as well as the methods of single molecule force spectroscopy (SMFS) and simultaneous topography and recognition imaging (TREC) will be introduced and discussed. Selected experiments will be presented in more detail to illustrate the combined application of these techniques in the elucidation of questions in molecular biology, pharmaceutical science and the medical field.

Original languageEnglish
Title of host publicationNanotribology and Nanomechanics
Subtitle of host publicationAn Introduction: Fourth Edition
PublisherSpringer
Pages135-173
Number of pages39
ISBN (Electronic)9783319514338
ISBN (Print)9783319514321
DOIs
Publication statusPublished - 1 Jun 2017

Keywords

  • Atomic force microscopy
  • Biomedical sensing
  • Recognition imaging
  • Single molecule force spectroscopy

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