Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching

Eljesa Murtezi; Sujitha Puthukodan; Jaroslaw Jacak; Thomas A. Klar

doi:10.1021/acsami.8b11777

Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching

Eljesa Murtezi, Sujitha Puthukodan, Jaroslaw Jacak, Thomas A. Klar

Research Center Linz

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Stimulated emission depletion (STED) nanolithography allows nanofabrication below the diffraction limit. Recently, it was applied to nanoanchors for protein fixation down to the single molecule level. We now combined STED nanolithography with laser-assisted protein adsorption by photobleaching (LAPAP) for optical and selective attachment of proteins to subdiffractional structures. In turn, STED was used for imaging of fluorescently tagged streptavidin to reveal protein binding to STED-lithographically patterned acrylate structures via LAPAP. Protein localization down to 56 nm spots was achieved using all-optical methods at visible wavelengths.

Original language	English
Pages (from-to)	31850-31854
Number of pages	5
Journal	ACS Applied Materials and Interfaces
Volume	10
Issue number	38
DOIs	https://doi.org/10.1021/acsami.8b11777
Publication status	Published - 26 Sept 2018

Keywords

biofunctionalization
laser-assisted protein adsorption by photobleaching
nanolithography
stimulated emission depletion
two-photon absorption
two-photon lithography
Nanotechnology/methods
Streptavidin/chemistry
Lasers
Protein Binding
Photobleaching
Polymers/chemistry

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10.1021/acsami.8b11777

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title = "Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching",

abstract = "Stimulated emission depletion (STED) nanolithography allows nanofabrication below the diffraction limit. Recently, it was applied to nanoanchors for protein fixation down to the single molecule level. We now combined STED nanolithography with laser-assisted protein adsorption by photobleaching (LAPAP) for optical and selective attachment of proteins to subdiffractional structures. In turn, STED was used for imaging of fluorescently tagged streptavidin to reveal protein binding to STED-lithographically patterned acrylate structures via LAPAP. Protein localization down to 56 nm spots was achieved using all-optical methods at visible wavelengths.",

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AU - Murtezi, Eljesa

AU - Puthukodan, Sujitha

AU - Jacak, Jaroslaw

AU - Klar, Thomas A.

PY - 2018/9/26

Y1 - 2018/9/26

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KW - biofunctionalization

KW - laser-assisted protein adsorption by photobleaching

KW - nanolithography

KW - stimulated emission depletion

KW - two-photon absorption

KW - two-photon lithography

KW - Nanotechnology/methods

KW - Streptavidin/chemistry

KW - Lasers

KW - Protein Binding

KW - Photobleaching

KW - Polymers/chemistry

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JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 38

ER -

Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching

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