Atomic force microscopy

M. Duman; I. Neundlinger; R. Zhu; J. Preiner; C. Lamprecht; L. A. Chtcheglova; C. Rankl; T. Puntheeranurak; A. Ebner; P. Hinterdorfer

doi:10.1016/B978-0-12-374920-8.00213-7

Atomic force microscopy

M. Duman, I. Neundlinger, R. Zhu, J. Preiner, C. Lamprecht, L. A. Chtcheglova, C. Rankl, T. Puntheeranurak, A. Ebner, P. Hinterdorfer

Research Center Linz

Research output: Chapter in Book/Report/Conference proceedings › Chapter › peer-review

1 Citation (Scopus)

Abstract

Atomic force microscopy (AFM) allows high-resolution topographical images to be obtained at the nanometer scale together with single molecule force measurements on biological samples in their physiological environment. Therefore, it is a powerful tool for detecting the forces and the dynamics of the interaction between individual ligands and receptors on cellular surfaces. This article describes sample preparation methods, different AFM imaging modes, AFM tip functionalization, current methodology, and applications of molecular recognition studies using AFM.

Original language	English
Title of host publication	Comprehensive Biophysics
Publisher	Elsevier
Pages	111-143
Number of pages	33
Volume	2
ISBN (Print)	9780080957180
DOIs	https://doi.org/10.1016/B978-0-12-374920-8.00213-7
Publication status	Published - 2012

Keywords

Atomic force microscopy
Cell fixation
Cell immobilization
Cellular membrane
Contact mode imaging
Dynamic mode imaging
Fluorescence microscopy
Force mapping
Intermittent (tapping) mode imaging
Magnetic AC mode imaging
Membrane channels
Membrane receptors
Molecular recognition force spectroscopy
Simultaneous topography and recognition imaging

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10.1016/B978-0-12-374920-8.00213-7

Cite this

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title = "Atomic force microscopy",

abstract = "Atomic force microscopy (AFM) allows high-resolution topographical images to be obtained at the nanometer scale together with single molecule force measurements on biological samples in their physiological environment. Therefore, it is a powerful tool for detecting the forces and the dynamics of the interaction between individual ligands and receptors on cellular surfaces. This article describes sample preparation methods, different AFM imaging modes, AFM tip functionalization, current methodology, and applications of molecular recognition studies using AFM.",

keywords = "Atomic force microscopy, Cell fixation, Cell immobilization, Cellular membrane, Contact mode imaging, Dynamic mode imaging, Fluorescence microscopy, Force mapping, Intermittent (tapping) mode imaging, Magnetic AC mode imaging, Membrane channels, Membrane receptors, Molecular recognition force spectroscopy, Simultaneous topography and recognition imaging",

author = "M. Duman and I. Neundlinger and R. Zhu and J. Preiner and C. Lamprecht and Chtcheglova, {L. A.} and C. Rankl and T. Puntheeranurak and A. Ebner and P. Hinterdorfer",

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language = "English",

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T1 - Atomic force microscopy

AU - Duman, M.

AU - Neundlinger, I.

AU - Zhu, R.

AU - Preiner, J.

AU - Lamprecht, C.

AU - Chtcheglova, L. A.

AU - Rankl, C.

AU - Puntheeranurak, T.

AU - Ebner, A.

AU - Hinterdorfer, P.

PY - 2012

Y1 - 2012

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AB - Atomic force microscopy (AFM) allows high-resolution topographical images to be obtained at the nanometer scale together with single molecule force measurements on biological samples in their physiological environment. Therefore, it is a powerful tool for detecting the forces and the dynamics of the interaction between individual ligands and receptors on cellular surfaces. This article describes sample preparation methods, different AFM imaging modes, AFM tip functionalization, current methodology, and applications of molecular recognition studies using AFM.

KW - Atomic force microscopy

KW - Cell fixation

KW - Cell immobilization

KW - Cellular membrane

KW - Contact mode imaging

KW - Dynamic mode imaging

KW - Fluorescence microscopy

KW - Force mapping

KW - Intermittent (tapping) mode imaging

KW - Magnetic AC mode imaging

KW - Membrane channels

KW - Membrane receptors

KW - Molecular recognition force spectroscopy

KW - Simultaneous topography and recognition imaging

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DO - 10.1016/B978-0-12-374920-8.00213-7

M3 - Chapter

AN - SCOPUS:84882787867

SN - 9780080957180

VL - 2

SP - 111

EP - 143

BT - Comprehensive Biophysics

PB - Elsevier

ER -

Atomic force microscopy

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Keywords

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